Chemical enhancement of survival in aggregation of retrovirus-infected rat cells: an interlaboratory comparison.
Chemical enhancement of survival in aggregation of retrovirus-infected rat cells: an interlaboratory comparison.
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逆转录病毒感染的大鼠细胞聚集中的化学增强存活：实验室间比较。
DOI：
10.1002/em.2860070511
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Tennant,RW
中科院分区：
文献类型：
--
作者：
Suk,WA;Poiley,JA;Raineri,R;Steuer,AF;Tennant,RW
本研究比较了三个独立实验室在逆转录病毒(Rauscher白血病)感染的Fischer鼠胚胎(RIFRE)细胞聚集存活试验中对16种化学物质的反应。当悬浮在琼脂基上的液体介质中时，对照细胞表现出细胞存活率的迅速下降，而以前用化学致癌物处理的细胞在悬浮液中存活的时间比对照细胞长。终点，聚集性存活，通过计数悬浮4天内从聚集物中解离的活细胞来衡量。通过修改以前报道的程序，我们改进了该系统，以便用已知致癌物或已知非致癌物处理的细胞获得明确的区分(阳性或阴性)反应。使用旨在最小化化验可变性的程序，执行重复化验，并分析数据以进行室内和室内一致性。RIFRE细胞聚集存活试验表明，在评估已知致癌物和非致癌物的总体阳性或阴性反应方面，实验室间具有高度的重复性，在评估按代码测试的化合物时，具有良好的定性重复性。该检测方法可以区分已知致癌物质和非致癌物质。所有的化学物质都在没有添加代谢激活系统的情况下进行了测试。表现出致癌物诱导的聚集存活增强的细胞，当被重新电镀到固体底物上并在培养中携带时，随后在其细胞形态、在半固体介质中的生长和在裸鼠体内的致瘤性方面表现出与转化相关的变化。这些结果表明，逆转录病毒感染的Fischer大鼠胚胎细胞检测到致癌物介导的早期事件，并进展为肿瘤表型。聚集生存似乎需要外源性逆转录病毒的存在，因为当比较致癌物质处理的细胞、非致癌物质处理的细胞或对照细胞时，未感染的细胞没有表现出不同的生存反应。该系统提供了一种可重复性的检测致癌化学物质的方法，该方法基于其诱导处理细胞聚集的提高存活率的能力。
This study provides a comparative evaluation among three independent laboratories of the responses to 16 chemicals in the retrovirus (Rauscher leukemia) infected Fischer rat embryo (RIFRE) cell‐survival‐in‐aggregation assay. When suspended in liquid media above an agar base, control cells showed a rapid decline in cell survival, whereas cells that had previously been treated with chemical carcinogen survive in suspension longer than control cells. The endpoint, survival in aggregation, is measured by counting viable cells dissociated from aggregates in suspension for 4 days. By modifying previously reported procedures, we have improved the system so that a clear differential (positive or negative) response is achieved by cells treated with either a known carcinogen or known noncarcinogen. Using procedures designed to minimize assay variability, replicate assays were performed and the data analyzed for inter‐ and intralaboratory concordance. The RIFRE cell‐survival‐in‐aggregation assay demonstrated a high degree of interlaboratory reproducibility in assessing the overall positive or negative responses of known carcinogens and noncarcinogens, and good qualitative reproducibility in assessing compounds tested under code. The assay could discriminate between known carcinogens and noncarcinogens. All chemicals were tested without the addition of a metabolic activation system. Cells exhibiting carcinogen‐induced enhancement of survival in aggregation, when plated back onto a solid substrate and carried in culture, subsequently expressed transformation‐associated changes in their cellular morphology, growth in semisolid media, and tumorigenicity in nude mice. These results indicate that retrovirus‐infected Fischer rat embryo cells detect a carcinogen‐mediated early event that progresses to neoplastic phenotypes. Survival in aggregation appears to require the presence of the exogenous retrovirus, since uninfected cells did not show a differential survival response when carcinogen‐treated, noncarcinogen‐treated, or control cells were compared. This system provides a reproducible method of detecting carcinogenic chemicals based on their ability to induce enhanced survival in aggregation of treated cells.
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通过类型特异性抗病毒抗体在体外预防病毒化学协同致癌作用。
DOI：
--
发表时间：
1976
影响因子：
11.1
作者：
P. Price;T. Bellew;M. P. King;A. Freeman;R. Gilden;R. Huebner
通讯作者：
R. Huebner
异种C型RNA病毒感染的大鼠细胞的化学转化及其病毒特异性抗血清的抑制。
DOI：
--
发表时间：
1977
影响因子：
11.1
作者：
P. Price;W. Suk;R. Peters;R. Gilden;R. Huebner
通讯作者：
R. Huebner
正常和转化的大鼠肝上皮细胞系以聚集形式表现出存活差异。
影响因子：
11.2
作者：
A. F. Steuer;P. Hentosh;L. Diamond;R. Ting
通讯作者：
R. Ting
核糖核酸酶 H：核糖核酸肿瘤病毒病毒粒子中普遍存在的活性
影响因子：
5.4
作者：
D. Grandgenett;G. Gerard;M. Green
通讯作者：
M. Green
3-甲基胆蒽和Rauscher白血病病毒联合作用对大鼠胚胎细胞的形态转化。
DOI：
--
发表时间：
1971
期刊：
Nature: New biology
影响因子：
--
作者：
Paul J. Price;Aaron E. Freeman;William T. Lane;Robert J. Huebner
通讯作者：
Robert J. Huebner