A nonstructural protein encoded by a rice reovirus induces an incomplete autophagy to promote viral spread in insect vectors.
A nonstructural protein encoded by a rice reovirus induces an incomplete autophagy to promote viral spread in insect vectors.
复制标题
DOI：
10.1371/journal.ppat.1010506
复制
发表时间：
2022-05
期刊：
影响因子：
6.7
通讯作者：
中科院分区：
文献类型：
--
作者：
病毒可以劫持自噬体作为培养的宿主细胞中的非溶解性释放载体。然而，自噬体介导的病毒传播如何在体内受感染的宿主组织或器官中发生仍然知之甚少。在这里，我们报告了一个重要的水稻呼肠孤病毒，水稻瘤矮病毒（RGDV）劫持自噬体穿越多个昆虫膜屏障中的中肠和唾液腺的叶蝉载体，以加强病毒的传播。这种含有病毒的双膜自噬体被阻止降解，导致病毒繁殖增加。从机制上讲，病毒非结构蛋白Pns 11诱导自噬并将自身嵌入自噬体膜中。自噬相关蛋白5（ATG 5）-ATG 12缀合对于初始的自噬体膜生物发生是必不可少的。RGDV Pns 11在体外和体内均与ATG 5特异性相互作用。ATG 5或Pns 11表达的沉默抑制ATG 8脂化、自噬体形成和有效的病毒繁殖。因此，Pns 11可以直接募集ATG 5-ATG 12结合以诱导自噬体的形成，从而促进病毒在昆虫体内的传播。此外，Pns 11可能通过直接靶向和介导溶酶体膜上N-糖基化Lamp 1的表达减少来阻断自噬体降解。总之，这些结果突出了RGDV如何重塑自噬体以利于其昆虫载体中的病毒繁殖。许多植物病毒在其昆虫载体的细胞内复制。在这里，我们证明了后代病毒粒子的水稻瘤矮病毒在叶蝉载体被吞噬的病毒诱导的双膜自噬体内。这种自噬体被修饰以逃避降解，因此可以被病毒持续利用以安全地运输病毒体穿过多个昆虫膜屏障。病毒非结构蛋白Pns 11通过与ATG 5相互作用诱导自噬体的形成，并通过介导溶酶体膜上N-糖基化Lamp 1的表达减少来潜在地阻断自噬体降解。这是我们第一次发现，一种由持久性植物病毒编码的非结构蛋白可以诱导不完全的自噬，以利于病毒在其昆虫载体中的繁殖。
Viruses can hijack autophagosomes as the nonlytic release vehicles in cultured host cells. However, how autophagosome-mediated viral spread occurs in infected host tissues or organs in vivo remains poorly understood. Here, we report that an important rice reovirus, rice gall dwarf virus (RGDV) hijacks autophagosomes to traverse multiple insect membrane barriers in the midgut and salivary gland of leafhopper vector to enhance viral spread. Such virus-containing double-membraned autophagosomes are prevented from degradation, resulting in increased viral propagation. Mechanistically, viral nonstructural protein Pns11 induces autophagy and embeds itself in the autophagosome membranes. The autophagy-related protein 5 (ATG5)-ATG12 conjugation is essential for initial autophagosome membrane biogenesis. RGDV Pns11 specifically interacts with ATG5, both in vitro and in vivo. Silencing of ATG5 or Pns11 expression suppresses ATG8 lipidation, autophagosome formation, and efficient viral propagation. Thus, Pns11 could directly recruit ATG5-ATG12 conjugation to induce the formation of autophagosomes, facilitating viral spread within the insect bodies. Furthermore, Pns11 potentially blocks autophagosome degradation by directly targeting and mediating the reduced expression of N-glycosylated Lamp1 on lysosomal membranes. Taken together, these results highlight how RGDV remodels autophagosomes to benefit viral propagation in its insect vector. Numerous plant viruses replicate inside the cells of their insect vectors. Here, we demonstrate that the progeny virions of rice gall dwarf virus in leafhopper vector are engulfed within virus-induced double-membraned autophagosomes. Such autophagosomes are modified to evade degradation, thus can be persistently exploited by viruses to safely transport virions across multiple insect membrane barriers. Viral nonstructural protein Pns11 induces the formation of autophagosomes via interaction with ATG5, and potentially blocks autophagosome degradation via mediating the reduced expression of N-glycosylated Lamp1 on lysosomal membranes. For the first time, we reveal that a nonstructural protein encoded by a persistent plant virus can induce an incomplete autophagy to benefit viral propagation in its insect vectors.
登录
查看更多内容
植物病毒诱导的自噬途径促进其昆虫载体的病毒传播和传播
影响因子：
6.7
作者：
Chen Y;Chen Q;Li M;Mao Q;Chen H;Wu W;Jia D;Wei T
通讯作者：
Wei T
DOI：
10.1038/s41579-018-0003-6
发表时间：
2018-06
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Choi Y;Bowman JW;Jung JU
通讯作者：
Jung JU
影响因子：
5.6
作者：
Ichimiya T;Yamakawa T;Hirano T;Yokoyama Y;Hayashi Y;Hirayama D;Wagatsuma K;Itoi T;Nakase H
通讯作者：
Nakase H
影响因子：
2.7
作者：
Moriyasu, Y.;Maruyama-Funatsuki, W.;Omura, T.
通讯作者：
Omura, T.
影响因子：
5.3
作者：
Dong X;Levine B
通讯作者：
Levine B