Identification and Characterization of Odorant Binding Proteins in the Forelegs of Adelphocoris lineolatus (Goeze).
Identification and Characterization of Odorant Binding Proteins in the Forelegs of Adelphocoris lineolatus (Goeze).
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Adelphocoris lineolatus (Goeze) 前腿气味结合蛋白的鉴定和表征
DOI：
10.3389/fphys.2017.00735
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发表时间：
2017
影响因子：
4
通讯作者：
Zhang YJ
中科院分区：
文献类型：
--
作者：
Sun L;Wang Q;Wang Q;Dong K;Xiao Y;Zhang YJ
昆虫的化学感受系统是昆虫感知外源物质的重要系统，气味结合蛋白（OBP）在气味结合和传递中起着重要作用。苜蓿盲蝽是多种作物的重要害虫，在苜蓿盲蝽中，我们对触角偏置OBP的生理作用的理解急剧增加，而与味觉相关的OBP大多未被探索。在这项研究中，我们采用RNA测序和RACE PCR方法，以确定推定的OBP从成年前肢的性别。确定了8个候选OBP，3个OBP（AlinOBP 15、16和17）为新的。全长序列比对和系统发育分析表明，这三个候选OBP具有昆虫OBP家族的典型特征。AlinOBP 16和17显示了6个高度保守的半胱氨酸，将它们置于经典的OBP亚家族中，而AlinOBP 15类似于AlinOBP 14，并与Plus-C进化枝聚类。定量实时PCR（qRT-PCR）揭示了不同的和显着的组织和性别偏见的表达模式。AlinOBP 15在雌虫头部高表达，AlinOBP 16和AlinOBP 17在雌虫触角中高表达。特别是，AlinOBP 11，在我们的前腿转录组数据集中最丰富的OBP基因，主要在成年腿中表达。此外，在成虫前肢上还观察到四种感器毛。lineolatus，包括刺形感器、刚毛感器和两种刺形感器（Sch 1和Sch 2）。抗AlinOBP 11抗血清可强烈标记Sch 2的外感器淋巴，表明它在A.线状的我们目前的研究结果提供的证据表明，OBP可以在半翅目盲蝽物种的跗味觉感受器的功能表达，拓宽了我们的理解OBP在昆虫中的化学感觉功能，并促进发现新的功能靶标调节昆虫寄主搜索行为。
The chemosensory system is essential for insects to detect exogenous compounds, and odorant binding proteins (OBPs) play crucial roles in odorant binding and transduction. In the alfalfa plant bug Adelphocoris lineolatus, an important pest of multiple crops, our understanding of the physiological roles of antenna-biased OBPs has increased dramatically, whereas OBPs related to gustation have remained mostly unexplored. In this study, we employed RNA sequencing and RACE PCR methods to identify putative OBPs from the adult forelegs of both sexes. Eight candidate OBPs were identified, and three OBPs (AlinOBP15, 16, and 17) were novel. Full-length sequence alignment and phylogenetic analyses suggested that these three candidate OBPs had characteristics typical of the insect OBP family. AlinOBP16 and 17 displayed six highly conserved cysteines, placing them in the classic OBP subfamily, whereas AlinOBP15 resembled AlinOBP14 and clustered with the Plus-C clade. Quantitative real-time PCR (qRT-PCR) revealed distinct and significant tissue- and sex-biased expression patterns. AlinOBP15 was highly expressed in female heads, and AlinOBP16 and 17 were strongly expressed in female antennae. In particular, AlinOBP11, the most abundant OBP gene in our foreleg transcriptome dataset, was predominately expressed in adult legs. Furthermore, four types of sensilla hairs were observed on the forelegs of adult A. lineolatus, including sensilla trichodea, setae, and two types of sensilla chaetica (Sch1 and Sch2). Anti-AlinOBP11 antiserum strongly labeled the outer sensillum lymph of Sch2, implying that it has important gustatory functions in A. lineolatus. Our current findings provide evidence that OBPs can be functionally expressed in the tarsal gustatory sensilla of hemipteran mirid species, broadening our understanding of OBP chemosensory function in insects and facilitating the discovery of new functional targets for the regulation of insect host-searching behaviors.
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