Studies on the Reaction Kinetics and Mechanisms of Enzymes Involved in the Biosynthesis of B-Lactam Antibiotics Using Purified Enzymes

本研究是关于β-内酰胺类抗生素生物合成相关酶的分离纯化、生化特性、反应动力学和反应机理的基础生化工程研究。了解参与生物合成β-内酰胺类抗生素的酶的总体生化特征和基本反应动力学及机理，并进一步将本研究获得的知识库应用于改进发酵工艺和/或开发新的和直接的体外酶工艺以生产现有的和新的β-内酰胺类抗生素衍生物。在研究的初始阶段，努力将重点放在那些参与β-内酰胺抗生素成环和扩环反应的关键酶的纯化和表征上。

This research is a fundamental biochemical engineering study on the purification, biochemical characterization, and the reaction kinetics and mechanisms of enzymes involved in the biosyntheses of beta-lactam antibiotics. The overall biochemical characteristics and basic reaction kinetics and mechanisms of those enzymes involved in the biosynthesis of beta-lactam antibiotics and further apply the knowledge base to be acquired from this research to the improvement of fermentation process and/or development of new and direct in-vitro enzymatic processes for production of existing and novel beta-lactam antibiotics derivatives. During the initial phase of the research, efforts will focus on the purification and characterization of those key enzymes involved in the ring formation and ring expansion reactions of beta-lactam antibiotics.