Acquisition of an Inverted Microscope with Video Camera and Time Lapse Recorder

该提案要求提供资金，用于购买蔡司倒置显微镜和配件。该显微镜将用于：L。评价培养的大鼠垂体细胞的细胞结构和分泌特性，以确定促性腺激素分泌细胞亚群在持续激素刺激期间是否被差异化地招募来释放激素。2.定量测定激活的人外周血单核细胞因子对内皮细胞形态和迁移行为的影响，3.鉴定和研究培养的心肌和血管平滑肌细胞上的离子通道分布，4.研究荧光染料在偶联心肌细胞间的转移，获得细胞形态计量学数据；5.评价含乙酰胆碱的前角神经元的功能特性。显微镜和附件将使生理系的教职员工受益，并将使该系扩大和拓宽其在电生理学方面的基础研究潜力。

This proposal requests funds for the purchase of a Zeiss inverted microscope and accessories. The microscope will be used to: l. Evaluate the cytostructural and secretory properties of cultured rat pituitary cells in order to determine whether subpopulations of gonadotropin secreting cells are differentially recruited to release hormone during periods of continuous hormonal stimulation, 2. Quantitate changes in endothelial cell morphology and migration behavior in response to activated human peripheral blood mononuclear cell factor, 3. Identify and study ion channel distributions on cultured myocardial and vascular smooth muscle cells, 4. Study the transfer of fluorescent dyes between coupled myocardial cells and to obtain cellular morphometric data, and 5. Evaluate the functional properties of acetylcholine containing ventral horn neurons. The microscope and accessories will benefit the faculty of the Department of Physiology and will allow the department to expand and broaden its basic research potential in electrophysiology.