High Performance Liquid Chromatography in an Integrated Advanced Laboratory Curriculum

曼哈顿学院和圣文森特山学院的本科理工科学生将使用高效液相色谱系统向本科生讲授高效液相色谱原理。该系统将用于四门综合的高级实验室课程。在这些课程中，学生发展分析、仪器、物理、合成和生化领域的技能和技巧。实验是按顺序进行的，因此首先学习基本技能，然后在最后的课程中逐步完成复杂的、面向项目的工作。该项目既涉及使用先前测试的方案，也涉及开发新的实验。他们的目标是向学生传授高效液相色谱的原理和应用。将使用几种特定的层析模式：正相、反相、离子交换和凝胶渗透。此外，还将使用几种类型的探测器：紫外/可见光可变波长吸光度、折射率、荧光和电化学。课程的设计是为了让学生在早期的实验中学习基本原理，而在后面的课程中，学生使用高效液相色谱作为研究诸如动力学等主题的工具。

A high performance liquid chromatography system will be used to teach principles of HPLC to undergraduate science and engineering students at Manhattan College and the College of Mount St. Vincent. The system will be used in four integrated, upper-division laboratory courses. In these courses, students develop skills and techniques in the analytical, instrumental, physical, synthetic, and biochemical areas. Experiments are sequenced so that basic skills are learned first, with a progression toward sophisticated, project-oriented work in the final course. The project involves both the use of previously tested protocols and the development of new experiments. Their object will be teaching students the principles and applications of HPLC. Several specific chromatographic modes will be used: normal phase, reverse phase, ion exchange, and gel permeation. In addition, several types of detectors will be used: UV/visible variable wavelength absorbance, refractive index, fluorescence, and electrochemical. The curriculum is designed so that fundamental principles are learned in the early labs, while students in the later courses use the HPLC as an investigative tool for topics such as kinetics.