Expression of Storage Protein in Somatic Embryos of Plants

苜蓿种子贮藏蛋白11S和14.5S抗体 莴苣种子贮藏蛋白在阶段I期间制备。 同相 II，PI建议使用这些抗体来确定 两种作物体细胞胚的发育阶段。 在苜蓿中，11S 在合子种子成熟期间测量贮藏蛋白， 在发芽过程中。 这将确定一个时间表， 可以比较体细胞胚的发育。 紫花苜蓿的条件 提出了体细胞胚培养，其导致非常高比率的 胚萌发 将对来自这些处理的体细胞胚胎进行测试 并确定了胚萌发与11S种子的相关性 蛋白质积累 控制早熟发芽的条件 体细胞胚将测试11S储存蛋白的损失， 紫花苜蓿 在莴苣中，合子的类似发育时间表 胚胎将建立。 细胞培养将于 建立胚繁殖的条件。 条件 将对14.5S蛋白进行研究。 该提案的总体目标是改善商业工厂 利用体细胞胚胎发生进行繁殖。 具体目标是 确定体细胞胚中的贮藏蛋白水平是否与 胚胎发育和活力。 在这个假设下，PI 预期通过增加内部细胞因子来改善体细胞胚胎发育 蛋白质储备

Antibody to the 11S seed storage protein of alfalfa and to the 14.5S seed storage protein of lettuce were prepared during Phase I. In Phase II, the PIs propose to use these antibodies to determine the developmental stage of somatic embryos of both crops. In alfalfa, 11S storage protein will be measured during zygotic seed maturation and during germination. This will determine a timetable against which the development of somatic embryos can be compared. Conditions of alfalfa somatic embryo culture are presented which result in very high rates of embryo germination. Somatic embryos from these treatments will be tested and a correlation determined between embryo germination and 11S seed protein accumulation. Conditions which control precocious germination of somatic embryos will be tested for the loss of 11S storage protein in alfalfa. In lettuce, a similar developmental timetable for zygotic embryos will be established. Lettuce cell cultures will be initiated in which conditions for embryo propagation will be established. Conditions for embryo maturation will be studied with respect to 14.5S protein. The overall objective of the proposal is to improve commercial plant propagation using somatic embryogenesis. The specific objective is to determine whether storage protein levels in somatic embryos correlate with embryo development and vigor. On this assumption, the PIs anticipate improving somatic embryo development by increasing internal protein storage reserves.