Differentiation of the Interstitial Cell Line in Hydrozoan Planulae

马丁博士将研究一种海洋水生动物Halocordyl disticha胚胎发生过程中未分化细胞（间质细胞）的发育和分化。浮藻含有大量的间质细胞，这些间质细胞要么分裂维持I细胞群，要么分化成神经节神经细胞或网状细胞。秋水仙碱和羟基脲处理的胚胎I细胞数量分别为零或减少。本文将分析I-细胞在幼虫组织中的五个主要方面的行为：1)幼虫干细胞在上皮planulae中的再生动态；2) i细胞及其衍生物参与变态；3)确定幼虫i细胞的发育潜力是否随着时间的推移而受到限制；4)研究抗羟基脲间质细胞幼虫群体的干细胞特性和发育潜力；5)幼虫种群与成虫种群的比较。将不同处理过或老化的对照胚胎块移植到上皮planulae上，并在移植后的不同时间取出进行分析。本研究探讨的一般问题是，在胚胎发生过程中，分化细胞的空间格局是如何建立的，以及这种组织如何在成年状态下被延续和维持。马丁博士正在研究以海洋水螅动物为模型的可操纵干细胞群的细胞承诺、增殖和分化。

Dr. Martin will examine the development and differentiation of undifferentiated cells, interstitial cells, during embryogenesis in a marine hydrozoan, Halocordyl disticha. Hydrozoan planulae contain a population of interstitial cells which either divide to maintain the I- cell population or differentiate into ganglionic nerve cells or nematocytes. Embryos treated with colchicine or hydroxyurea have either no or reduced numbers of I cells respectively. Five major aspects of I- cell behaviour in larval tissues will be analyzed: 1) the dynamics of larval stem cell repopulation in epithelial planulae; 2) the involvement of I-cells and their derivatives in metamorphosis; 3) a determination if larval I-cells exhibit restrictions in their developmental potential with time; 4) an examination of the stem cell properties and developmental potential of a larval population of hydroxyurea resistant interstitial cells; and 5) a comparison of the larval I-cell population to the adult I-cell population. Pieces of different treated or aged control embryos will be grafted to epithelial planulae and removed at various times after grafting for analysis. The general problem approached by this research is how the spatial pattern of differentiated cells is established during embryogenesis and how such an organization is carried over and maintained in the adult state. Dr. Martin is studying cellular commitment, proliferation and differentiation of a manipulatable population of stem cells in a marine hydrozoan as a model.