Visualising chromatin changes in 3 dimensions: super to ultra resolution

将基因组信息打包到细胞核中需要在细胞核内形成紧密致密和高度组织的基因组结构，这种结构天生就是对基因转录的抑制。因此，改变DNA空间组织的机制对于实现包括DNA转录在内的各种基因组功能至关重要。这项提议将利用超分辨率显微镜的新适应方式，在3个维度上可视化染色质修饰的变化如何影响细胞核内的基因组空间组织，以及这如何与细胞分化联系起来。这将提供一幅图，说明核内的空间组织如何支持一般细胞分化。

Packaging of genomic information into the nucleus of a cell necessitates the formation of tightly compacted and highly organized genomic structures within the nucleus, a configuration that is inherently repressive for gene transcription. Hence, mechanisms that alter the spatial organisation of DNA are critical to enable a variety of genome functions, including DNA transcription. This proposal will utilise novel adaptations of super resolution microscopy to visualise in 3 dimensions how changes in chromatin modifications impact genome spatial organisation within the nucleus, and how this then links to cellular differentiation. This will provide a picture of how spatial organisation within the nucleus supports general cell differentiation.