Purchase of a High Mass High Performance Tandem Mass Spectrometer

该合同将为购买高质量、高性能串联质谱仪提供部分资金。该机器将用于氨基酸序列的测定，蛋白质转录后修饰的表征和交联配体-受体复合物的结构表征，合成多肽和其他生物感兴趣的化合物。要检查的蛋白质包括膜结合受体、激素、酶和转录因子。购买仪器所需的剩余资金将由学校提供。质谱法是一种越来越实用的技术，在各种各样的生化化合物的结构表征。特别令人感兴趣的是该技术在修饰蛋白、肽聚糖和其他多糖的序列分析中的应用，因为这些信息通常很难或不可能通过其他方法获得。该仪器的计划用途将导致分析方法的改进，并提供有关特定分子的重要新信息。

This award will provide partial funding for the purchase of a high mass, high performance tandem mass spectrometer. The machine will be used for determination of amino acid sequences, characterization of post-transcriptional modifications of proteins and structural characterization of cross-linked ligand-receptor complexes, synthetic polypeptides and other compounds of biological interest. Among the proteins to be examined are membrane-bound receptors, hormones, enzymes and transcription factors. The remaining funds neccessary for purchase of the instrument will be provided by the university. Mass spectrometry is a technique of increasing utility in the structural characterization of a wide variety of biochemical compounds. Of particular interest is the use of this technique in the sequence analysis of modified proteins, peptidoglycans and other polysaccharides, since this information is often difficult or impossible to obtain by other means. The planned uses for this instrument should result in improved analytical methods as well as providing important new information about particular molecules.