High-performance mass spectrometer for the analysis of clinical samples and model organisms

基于质谱的蛋白质组学已成为分子和医学研究的关键技术。为了分析复杂的生物样品，高灵敏度和高质量精度的质谱仪是必不可少的。另一个重要的要求是非常快的测序速度，这主要是通过四极杆、高能碰撞解离（HCD）和高分辨率高频检测器的组合来实现的。通过使用这种高分辨率的质谱，可以深入分析来自人体体液和模式生物的复杂蛋白质组及其修饰。因此，四极混合质谱仪（MS）的收购将成为科隆校区医学和自然科学研究小组的科学项目的重要资产。此外，由于样品数量多，因此将快速高效的LC系统与质谱仪耦合起来非常重要。额外的离线LC系统在这里提供了决定性的优势，因为样品和梯度的加载时间非常短，并且与经典的纳米LC- ms系统相比，每天的样品数量显着增加。新开发的离子迁移系统场不对称离子迁移谱法（FAIMS）代表了肽与可能的杂质的分离，将进一步提高仪器的灵敏度和准确性。由于新的研究小组的招募，科隆校区对定量蛋白质组学的需求大幅增加，一种新的最先进的质谱仪器对于保持高质量的质谱分析和增加CECAD/CMMC蛋白质组学设施的容量至关重要。

Mass spectrometry-based proteomics has become a key technology for molecular and medical research. To analyze complex biological samples, a mass spectrometer (MS) with high sensitivity and high mass accuracy is absolutely essential. Another important requirement is the very fast sequencing speed, which was mainly achieved by the combination of the quadrupole, higher energy collisional dissociation (HCD) and a high resolution-high frequency detector. By using this high-resolution MS, complex proteomes and their modification from human body fluids and model organisms can be analyzed in depth. The acquisition of a quadrupole hybrid mass spectrometer (MS) will therefore be an important asset for the scientific projects of the medical and natural science research groups at the Cologne Campus. Furthermore, due to the high number of samples, it is important to couple a fast and efficient LC system with the mass spectrometer. The additional offline LC system offers a decisive advantage here, as the loading times of the samples and gradients are very short and the number of samples per day is significantly increased compared to classical nano-LC-MS systems. The newly developed ion mobility system Field Asymmetric Ion Mobility Spectrometry (FAIMS) represents a separation of peptides from possible impurities and will further increase the sensitivity and accuracy of the instrument. As the demand for quantitative proteomics at the Cologne Campus has massively increased due to the recruitment of new research groups, a new state-of-the-art MS instrument is essential to maintain the high quality of MS analyses and to increase the capacity of the CECAD/CMMC proteomics facility.