Collaborative Research in Maize Genetics

这个奖项给爱荷华州州立大学将资助购买 有效参与所需的小型设备 Lee博士和Wendel博士与大卫进行合作研究项目 密苏里州大学的霍伊辛顿和艾德科（NSF 8721921）。 该项目将继续并扩大合作研究， 制作一个完整的玉米物理和遗传图谱。 将使用几种类型的遗传作图策略， 识别和关联RFLP标记的位置，细胞学 标记（旋钮，缺失，插入和倒位），生物化学 标记（负责同工酶的突变）和其他， 常规标记。 虽然大量的位置 这些不同类型的标记的信息现在是可用的， 相对而言，很少有人将 细胞学标记与生化标记、RFLP 地图与细胞学地图等。特别关注的是 应用新开发的RFLP作图技术， 地图还没有完成，因为还没有一个实验室 组装了一套完整的RFLP标记DNA，可用于 所有研究人员。 虽然这项研究将在 主要由Coe和霍伊辛顿博士，与其他 玉米遗传学家，特别是那些熟练的鉴定 生化标志物，是必需的 因此，小并发 爱荷华州的这些调查人员获得了奖励， 以及伊利诺斯州州立大学和北卡罗来纳州的其他大学 状态 这些奖项将提供实验室设备， 便利分析和比较在 独立的实验室，并将确保研究材料开发的 每一位调查员都将随时与所有 合作者 传统上，遗传作图技术一直是一种 一个有用的工具，研究人员感兴趣的各种基本 生物学问题以及植物和动物育种者。 最近发展的一种强大的新技术，称为RFLP 映射将对 遗传学可以在基础和应用方面发挥作用。 问题 然而，重要的是， 更传统的技术被保留下来， 与新的RFLP图谱整合（或相关）。 这是 尤其是玉米， 广泛的基因研究。 建议的合作研究 将加速开发完整的玉米RFLP图谱， 将确保地图是所有研究人员可用的工具 和农学家。 此外，通过促进 RFLP数据与用其他类型的遗传标记获得的数据， 这项研究将确保RFLP图谱的最大效用 给需要的人

This award to Iowa State University will fund the purchase of small equipment items necessary for the effective participation of Drs. Lee and Wendel in a collaborative research project with David Hoisington and Ed Coe at the University of Missouri (NSF 8721921). The project will continue and expand collaborative research aimed at producing an integrated physical and genetic map of maize. Genetic mapping strategies of several types will be used to identify and correlate the positions of RFLP markers, cytological markers (knobs, deletions, insertions and inversions), biochemical markers (mutations responsible for isozymes) and other, conventional markers. Although a substantial amount of positional information for these various types of markers is now available, relatively little has been done to correlate the map of cytological markers with that of the biochemical ones, the RFLP map with the cytological map and so on. Particular focus is placed on the newly developed technique of RFLP mapping because the map is not yet complete, and because no single lab has yet assembled a complete set of RFLP marker DNAs that are available to all researchers. Although the research will be performed principally by Drs, Coe and Hoisington, collaboration with other corn geneticists, especially those skilled in the identification of biochemical markers, is required. Thus, small concurrent awards are being made to these investigators at Iowa State, as well as others at Illinois State University and North Carolina State. These awards will provide laboratory equipment that will facilitate analysis and comparison of data developed in the separate labs and will insure that research materials developed by each investigator will be readily accessible to all the collaborators. Traditionally, techniques for genetic mapping have been a useful tool for researchers interested in a variety of basic questions in biology as well as for plant and animal breeders. The recent development of a powerful new technique called RFLP mapping will have a significant effect on the ease with which genetics can be brought to bear on both basic and applied problems. However, it is important that the knowledge and tools of the more traditional techniques be preserved and that they be integrated (or correlated) with the new RFLP maps. This is particularly true in the case of corn, which has been the subject of extensive genetic study. The proposed collaborative research will accelerate the development of a complete RFLP map of corn and will insure that the map is a tool available to all researchers and agronomists. Moreover, by facilitating the integration of the RFLP data with that obtained with other types of genetic markers, the research will insure that the RFLP map is of maximum utility for those who need it.