Acquisition of a 9.4 Tesla Magnet for Solid State NMR

为装配采购宽口径9.4特斯拉磁体 提出了一种固体核磁共振谱仪的设计方案。 本研究 是针对结构和动态的 蛋白质复合物（胶原）、蛋白质的表征 脂质复合物（脂质双层中的短杆菌肽A）和DNA 液晶相 文书的进一步使用 将由个人在广泛的学科， 时间允许的话。 固态核磁共振开始在 生物物理学，特别是在动力学领域。 另 动态表征的方法并不产生相同的结果， 这是固态核磁共振所能做到的细节。 是 可以从特定位点标记的生物分子来确定 一个具有原子分辨率的分子运动模型 这 模型包括对轴的描述， 运动正在发生，运动的类型，无论是 扩散的或不连续的，运动的幅度和 最后是运动的频率。 与这些 的动态特性的潜力 获得动力学和功能之间的相关性是非常重要的。 高 本文所述的许多具体实验将不 在目前相对较低的磁场（4.7特斯拉）上是可能的 固态核磁共振仪器在FSU设施，不仅 因为磁场很低， 有限的时间。一个详细的理由， 基于改进的高场（9.4T）仪器 灵敏度（玻尔兹曼分布），提高分辨率（通过 最小化场依赖14 N谱线展宽效应， 13C谱），低旋磁比观测 核，减少声振铃（2 H光谱），和 自旋非整数的四极核的观测

Acquisition of a wide bore 9.4 Tesla magnet for the assembly of a solid state NMR spectrometer is proposed. This research is directed toward the structural and dynamic characterization of protein complexes (collagen), protein lipid complexes (Gramicidin A in a lipid bilayer) and DNA liquid crystalline phases. Further use of the instrument will be made by individuals in a wide range of disciplines as time permits. Solid state NMR is beginning to play a major role in biophysics, especially in the realm of dynamics. The other approaches to dynamic characterizations do not yield the same sort of detail that is possible with solid state NMR. It is possible from specific site labeled biomolecules to determine a model for molecular motions with atomic resolution. This model includes a description of the axis about which the motion is occurring, the type of motion, whether it be diffusional or discontinuous, the amplitude of the motion and finally the frequency of the motion. With such characterizations of the dynamics the potential for obtaining correlations between dynamics and function is very high. Many of the specific experiments described herein would not be possible on the present relatively low field (4.7 Tesla) solid state NMR instrumentation in the FSU facility, not only because of the low field but also because of the severely limited time available on it. A detailed justification for a high field (9.4 Tesla) instrument is given, based on improved sensitivity (Boltzman distribution), improved resolution (by minimizing field dependent 14 N line broadening effects on the 13 C spectra), observation of low gyromagnetic ratio nuclei, reduced acoustic ringing (for 2 H spectra), and observation of quadrupolar nuclei with nonintegral spin.