Immobilized Metal Affinity (IMA) Membranes

本项目将研究固定化金属的应用 用于蛋白质分离和回收的亲和膜。 一 偶联化学将被开发用于连接IDA- 亚氨基二乙酸酯和TED-三（羧甲基）乙二胺 与间隔臂无机，多孔玻璃微滤 膜。 离子Cu（II）、Zn（II）和 将测量Ni（II）以确定结合常数， 在洗涤期间的金属离子稳定性。 改性膜 将用于分离单一和混合蛋白质 使用模型蛋白质系统。 梯度和逐步洗脱 吸附的蛋白质将尝试通过改变pH，使用 置换剂和使用螯合剂。 固定化金属亲和色谱法正发展成为 最有效的净化技术之一 生物技术产品。 膜的快速发展 技术也极大地改变了 生物技术的商业化。 的组合 这两种技术可能对 大规模蛋白质纯化技术的发展。

This project will investigate the use of Immobilized Metal Affinity Membranes for protein separation and recovery. A coupling chemistry will be developed for attaching IDA- iminodiacetate and TED-tris(carboxylmethyl) ethylenediamine with spacer arms to inorganic, porous glass microfiltration membranes. Loading isotherms for the ions Cu(II), Zn(II), and Ni(II) will be measured to determine binding constants and metal-ion stability during washing. The modified membranes will be used for fractionation of single and mixed proteins using model protein systems. Gradient and stepwise elution of adsorbed proteins will be attempted by varying pH, using displacers, and using a chelating agent. Immobilized metal affinity chromatography is developing into one of the most powerful techniques for purification of products from biotechnology. Rapid advances in membrane technology are also dramatically changing the engineering aspects of commercial scale biotechnology. The combination of these two techniques can have a potentially large effect on development of large-scale protein purification technologies.