Manipulating Metabolism for Enhanced Protein Synthesis

该项目的目标是开展一项基本的 细胞操作的定量研究 促进外源蛋白合成的代谢。 拟议的研究计划将使用以下组合： 实验和理论方法来研究 诱导蛋白表达对氨基酸的影响 调节和细胞生长。 这项研究将使用一个 用于诱导合成的表达载体 同源多肽，便于详细检查 胞内氨基酸耗竭。 另外这款 研究将检查代谢和产物表达 动态特性，使用专门设计的 在线细胞质量测量装置 动态代谢模型

The objective of this project is to undertake a fundamental and quantitative study of the manipulation of cell metabolism for the enhanced synthesis of foreign proteins. The proposed research program will use a combination of experimental and theoretical methods to examine the influence that induced protein expression has on amino acid regulation and cell growth. This study will use an expression vector for the induced synthesis of a homopolypeptide, facilitating a detailed examination of intracellular amino acid depletion. In addition, this research will examine metabolic and product expression characteristics dynamically, using a specially designed on-line cell mass measurement device in concert with a dynamic metabolic model.