Acquisition of a System for Quantitative Fluorescence Microscopy and Imaging at Florida State University

要求美国国家科学基金会提供资金，为生物科学部的六个研究小组使用显微镜成像系统提供便利，以增强仪器的性能。定量荧光系统将使研究人员能够研究各种新问题，包括：钙在垂体细胞分泌催乳素中的作用，软体动物肌肉细胞内pH的调节，钙通量在衣原体基因表达调控中的作用，关键蛋白在刷状缘细胞中的分布和潜在的区划，肌纤维中细胞内镁的动态变化，最后是细胞内pH在阿米巴细胞运动中的作用。收购定量荧光成像系统将对这六个集团产生重大影响，这六个集团都是外部资助的。

NSF funds are requested to purchase enhancements to the instrument to facilitate the use of a microscope imaging system by six research groups in the Department of Biological Science. The quantitative fluorescence system will allow the researchers to investigates a variety of new problems including the following: the role of calcium in prolactin secretion by pituitary cells, the regulation of the intracellular pH in molluscan myocytes, the role of calcium fluxes in the regulation of gene expression in Chlamydonas, the distribution and potential compartmentation of key proteins in brush border cells, the dynamics of intrecellular magnesium in muscle fibers and finally the role of intracellular pH in motility in amoeboid cells. The acquisition of the quantitative fluorescence imaging system will have significant impact on these six groups, all of which are externally funded.