Biosynthesis of Secondary Metabolites from Blue-Green Algae

这项研究将解决几个具体领域的中学 蓝藻代谢物的生物合成。第一组 待研究的化合物包括有效的 来自水华鱼腥藻的抗胆碱酯酶类毒素a， 肝毒性环肽微囊藻毒素-LR 铜绿假单胞菌和产泡沫节球藻的节球藻毒素， 神经毒性生物碱类毒素A和高类毒素A 阿氏颤藻和炎性因子林贝毒素 大鞘丝藻中的脱溴阿氏藻毒素。研究还 将进行隔震、结构确定 以及蓝藻中新毒素的生物合成。第二 一个主要领域将侧重于继续研究 杀真菌吲哚生物碱hapalindole A生物合成 和甲藻毒素、紫茉莉烯A 异腈、米拉比德E和坦他唑B， 四类结构独特和多样的自然 产品Scytonema mirabile。第三个领域的研究将 涉及由Cylindrocyclophane A的生物合成， Licheniforme，the first ım，n！对环芳烷 和一种细胞外物质， 刺激C.苔藓状。最后，研究将 进行隔离、结构测定， 从蓝绿色生物合成其他不寻常的天然产物 藻类，例如聚胞菌素，一种来自海洋的抗病毒化合物 蓝绿藻Synechococcus elongatus的新生物碱 属于针茅目的蓝藻。 %%% 凭借这一奖项，化学合成有机计划 该部门将支持理查德·E.摩尔 马诺阿夏威夷大学化学系。 研究的主要目的是了解 蓝藻中次级代谢产物的生物合成， 尤其是淡水、咸水和海洋蓝藻 产生的毒素与野生动物、家畜 和人类中毒

This research will address several specific areas of secondary metabolite biosynthesis in blue-green algae. The first group of compounds to be investigated includes the potent anticholinesterase anatoxin-a(s) from Anabaena flos-aquae, the hepatotoxic cyclic peptides microcystin-LR from Microcytis aeruginosa and nodularin from Nodularia spumigena, the neurotoxic alkaloids anatoxin-a and homoanatoxin-a from Oscillatoria agardhii, and the inflammatory agents lyngbyatoxin A and debromoaplysiatoxin from Lyngbya majuscula. Studies also will be carried out on the isolation, structure determination and biosynthesis of new toxins from cyanophytes. The second major area will focus on a continuation of studies on the biosynthesis of hapalindole A, a fungicidal indole alkaloid from Hapalosiphon fontinalis, and tolytoxin, mirabilene A isonitrile, mirabimide E, and tantazole B, representatives of four classes of structurally unique and diverse natural products from Scytonema mirabile. Studies in a third area will involve the biosynthesis of cylindrocyclophane A from Cylindrospermum licheniforme, the first ım,n!paracyclophane to be found in Nature, and an extracellular substance which stimulates sporulation in C. licheniforme. Finally studies will be carried out on the isolation, structure determination, and biosynthesis of other unusual natural products from blue-green algae, e.g. synechocin, an antiviral compound from the marine cyanophyte Synechococcus elongatus, and new alkaloids from cyanophytes belonging to the Stigonematales. %%% With this award the Synthetic Organic Program of the Chemistry Division will support the research of Dr. Richard E. Moore of the Department of Chemistry at the University of Hawaii, Manoa. The major goal of the research is to understand the biosynthesis of secondary metabolites in blue-green algae, especially freshwater, brackish-water, and marine cyanophytes which produce toxins implicated in wildlife, domestic animal, and human poisonings.