Stable Carbon and Nitrogen Isotopic Compositions of SelectedOrganic Compounds

将测量大块有机物、脂质和选定的色素（叶绿素a、b和c、b -胡萝卜素和色素）的稳定碳和氮同位素组成，以研究选定海洋环境中有机物的来源和循环。制备级高效液相色谱方法将用于分离足够数量的纯母体色素，用于精确和准确的稳定同位素分析。碳氮的吸收和生物合成利用引起了特征的动力学分馏，可以用来定义特定有机池的“生活史”。虽然大量有机物性质的测定是有用的，但特定标记化合物的稳定同位素分析对于多来源和复杂过程产生的有机基质的反卷积具有很大的希望。实地研究将包括表征三种明确界定的海洋环境，以评估色素特定稳定同位素分布的变异性、效用和局限性，用于(1)解析陆地和海洋生物量；(2)区分沉积物有机质来源；(3)确定“新”与“新”的相对重要性。上层水柱的“循环”生产。//

Stable carbon and nitrogen isotope compositions of bulk organic matter, lipids and selected pigments (chlorophylls a, b and c; B-cartene; and phaeopigments) will be measured to investigate the sources and cycling of organic matter in slected marine environments. Preparative-scale high-performance liquid chromatographic methods will be used to isolate sufficient quantities of pure parent pigments for precise and accurate stable isotope analysis. Carbon and nitrogen uptake and biosynthetic utilization cause characteristic kinetic fractionations that can be used to define the "life histories" of of specific organic pools. While determinations of bulk organic matter properties are useful, the stable isotopic analysis of specific marker compounds holds great promise for deconvoluting organic matrices produced by multiple sources and complex processes. Field studies will include the characterization of three well- defined oceanographic settings to evaluate the variability, utility and limitations of pigment-specific stable isotope distributions for (1) resolving terrestrial and marine biomass; (2) differentiating organic matter sources in sediments; and (3) determining the relative importance of "new" vs. "recycled" production in the upper water column. //