Utilization of Unnatural Amino Acids in de novo Protein Synthesis

这项研究将开发一种新的蛋白质 基于超次级组装的合成 结构基序通过掺入 特别设计的非天然氨基酸残基。 的合成、掺入和结构表征 多肽中的非天然氨基酸残基是至关重要的 研究的要素。 残留物将用作 约束β型II反向转弯的基础。 此夕h 非天然氨基酸残基应该有助于建立 通过提供更多的选择， β-支链氨基酸残基比目前 available. 该研究建议利用超级中学 （而不是三级）结构元素作为基本的 构建蛋白质设计的模块， 和分析过程。 的进一步功能化 非天然残基可以形成产生新的 调节或催化生物聚合物。 %%% 芭芭拉博士获得了有机合成项目的这一奖项 加州化学系的因佩里亚里 技术研究所将在该领域进行研究， 新蛋白质合成。 该研究将集中于开发 可以以合理的方式组装的积木， 提供具有预定的 三维结构。 因佩里阿里医生会用肽基 基于非天然氨基酸残基的化合物，其可以 用来制造新的蛋白质 的进一步功能化 非天然的残基可以导致产生新的调节性蛋白质， 或催化生物聚合物。

The research will develop an approach to de novo protein synthesis which is based on the assembly of supersecondary structural motifs stabilized by the incorporation of specifically designed unnatural amino acid residues. Synthesis, incorporation and structural characterization of unnatural amino acid residues within polypeptides are crucial elements of the research. The residues will be used as the basis for constrained beta-type II reverse turns. Further, the unnatural amino acid residues should help in establishing beta-sheet stucture by providing a greater choice of beta-branched amino acid residues than that currently available. The research proposes utilization of supersecondary (rather than tertiary) structural elements as the basic building blocks in protein design to facilitate both predictive and analytical processes. Further functionalization of the unnatural residues can form the basis for the production of new regulatory or catalytic biopolymers. %%% With this award from the Synthetic Organic Program, Dr. Barbara Imperiali of the Department of Chemistry at the California Institute of Technology will conduct research in the area of de novo protein synthesis. The research will focus on developing building blocks which can be assembled in a rational manner to afford proteinaceous products with a predetermined three-dimensional structure. Dr. Imperiali will use peptidyl compounds, based on unnatural amino acid residues, which can be used to build new proteins. Further functionalization of the unnatural residues can lead to the production of new regulatory or catalytic biopolymers.