Small Grants for Exploratory Research: Separation of Cells and Biological Macromolecules by Antibody Receptor Coated Magnetic Vesicles and Ferritin Conjugates

本课题探索了一种新的免疫磁性方法， 有效地将细胞或生物大分子从 混合物. 不 该方法使用带有抗体Fc受体的囊泡 并将磁性纳米颗粒封装在其中 水囊泡核心。 然后囊泡结合 选择性地作用于附着在特定细胞上的抗体， 然后磁性分离。 磁性囊泡 从细胞中分离出来，可以被“分离”。 第二 使用与Fc受体缀合的铁蛋白分子的方法， 而不是具有Fc受体的囊泡，也被探索用于 对比 绵羊红细胞的模型系统和 成功分离出鸡红细胞。 的 结合机理研究了光和电子 显微镜 探索了各种分离方案。 的 结果可能适用于临床分离- 例如来自健康细胞的癌细胞，-生物医学研究， 和生物技术。

This project explores a new immunomagnetic method for rapidly and efficiently separating cells or biological macromolecules from a mixture. T he method uses vesicles with antibodies Fc-receptors attached on them and with magnetic nanoparticles encapsulated within the aqueous vesicles cores. The vesicles then bind selectively on the antibodies attached to specific cells, which are then separated magnetically. The magnetic vesicles are separated from the cells and can be 'recycled'. A second method using ferritin molecules conjugated with Fc-receptors, rather than vesicles with Fc-receptors, is also explored for comparison. A model system of sheep red blood cells and chicken red blood cells has been successfully separated. The mechanism of binding is investigated by light and electron microscopy. Various separation schemes are explored. The results are potentially applicable to clinical separations - e.g. cancer cells from healthy cells, - biomedical research, and biotechnology.