,.S.-Japan Collaborative Research: Production and application of chromosome deletion stocks in genome mapping and meiotic paring analysis in common wheat

该奖项支持美国-日本合作研究计划 植物病理学系的Bikram Gill教授 堪萨斯州立大学教授， 奈良大学理学部。 这个为期两年的项目， 也涉及教授拉马科塔的堪萨斯州立大学，将 使用遗传诱导缺失作图的方法， 构建个体综合细胞遗传学图谱 普通小麦的染色体 染色体缺失是 是由某些山羊草属的染色体加入到小麦中引起的。 这些所谓的“杀配子”染色体将被用来 构建独特的缺失原种。 多基因缺失作图 试验原种由形态学、生理学、蛋白质、 和DNA标记位点的研究。 大小和 缺失染色体中的断裂点将通过C- 显带和原位杂交技术，以确定 特定遗传标记的物理位置。 这是NSF的一项免费提案。 它是必需的，因为 日本向日本促进和平协会提出的对应建议 促进科学（JSPS）要求为日本提供资金 investigators. 该奖项将联合收割机的研究兴趣， 美国研究人员对小麦基因组进行物理作图 随着日本调查人员对生产的研究， 删除股票。

This award supports a U.S.-Japan cooperative research project between Professor Bikram Gill, Department of Plant Pathology, Kansas State University, and Professor Takashi Endo, Institute of Natural Science, Nara University. This two-year project, which also involves Professor Rama Kota of Kansas State University, will use the approach of genetically-induced deletion mapping to construct comprehensive cytogenetic maps of individual chromosomes of common wheat. The chromosome deletions are caused by certain Aegilops chromosomes when added to wheat. These so-called "gametocidal" chromosomes will be used to construct unique deletion stocks. Deletion mapping with multigenic tester stocks consisting of morphological, physiological, protein, and DNA marker loci will also be undertaken. The size and breakage-point in deletion chromosomes will be identified by C- banding and in situ hybridization techniques in order to determine the physical location of specific genetic markers. This is a no-cost proposal for NSF. It is required because the Japanese counterpart proposal to the Japan Society for the Promotion of Science (JSPS) requests funding for the Japanese investigators. The award will combine the research interests of the U.S. investigators on physical mapping of the genome of wheat with the research of the Japanese investigators on the production of deletion stocks.