Application of In Vivo Mapping Strategies to Order Arabidopsis YAC Libraries

该奖项的目的是利用酵母人工染色体（YAC）载体分离、鉴定和绘制拟南芥基因组的大片段，并构建新的拟南芥YAC文库。本研究利用新开发的脉冲场凝胶施胶方法和酵母转化方法，对含有比现有文库更大平均插入的拟南芥YAC克隆进行了富集。新的、更大的文库应该将染色体行走实验的步骤数减少两倍以上，从而促进对感兴趣的特定基因的识别。这项研究的效用在于迅速向整个科学界分发和传播图书馆。Drs。Chory和Hoekstra将建立克隆存档和数据存储的方法，对图书馆进行编目，并与马萨诸塞州总医院的Howard Goodman小组合作，将现有的标记放置到已知的克隆上。YAC图书馆的副本将通过俄亥俄州立大学拟南芥生物资源中心分发给感兴趣的研究人员。

The objective of this award is the isolation, identification, and mapping of large segments of the Arabidopsis genome using yeast artificial chromosome (YAC) vectors and the construction of new Arabidopsis YAC libraries. This research takes advantage of newly developed pulse field gel sizing methods and yeast transformation approaches to enrich for Arabidopsis YAC clones that contain larger average inserts than are present in the existing libraries. The new, larger libraries should reduce the number of steps in a chromosome walking experiment by greater than a factor of two, thus facilitating the identification of specific genes of interest. The utility of the research lies in rapid distribution and dissemination of the library to the entire scientific community. Drs. Chory and Hoekstra will establish methods for clone archiving and data storage to catalog the library, and to place existing markers onto known clones in collaboration with the Howard Goodman's group at Massachusetts General Hospital. Copies of the YAC library will be distributed to interested researchers through the Arabidopsis Biological Resource Center at Ohio State University.