Circular Dichroism Spectrometer

路易斯维尔大学（University of Louisville）的一组研究人员申请资助圆形二色谱仪的一半费用，该光谱仪将用于评估溶液中的肽和蛋白质二级结构，用于研究温度、溶剂、pH值或配体结合变化引起的蛋白质构象变化。用于合成多肽和重组蛋白的构象分析。建议的具体项目包括将圆二色性应用于以下系统：(1)纯化的可溶性细胞色素P-450同工酶，以及nadph -细胞色素P-450还原酶的膜锚定肽，(2)参与外源化合物代谢的细胞质和微粒体酶，(3)重组人成纤维细胞前胶原酶和胶原酶，(4)天然RNA加工酶和经定点突变改变的重组酶，(5)线粒体靶向信号肽，(6) Ca++诱导的嗜铬粒蛋白A构象变化；(7)含有酰胺键取代的合成肽（假肽）。

A group of researchers at the University of Louisville requests funding for half of the cost of a circular dichroism spectrometer, which will be used for assessing peptide and protein secondary structure in solution, for investigating changes in protein conformation induced by alterations in temperature, solvent, pH, or ligand binding, and for conformational analysis of synthetic polypeptides and recombinant proteins. The specific projects proposed include application of circular dichroism to the following systems: (1) purified, soluble cytochrome P-450 isozymes, and the membrane anchoring peptide of NADPH-cytochrome P-450 reductase, (2) cytoplasmic and microsomal enzymes involved in the metabolism of foreign compounds, (3) recombinant human fibroblast procollagenase and collagenase, (4) native RNA processing enzymes and recombinant enzymes altered by site-directed mutagenesis, (5) mitochondrial targeting signal peptides, (6) Ca++ -induced conformational changes in chromogranin A, and (7) synthetic peptides containing amide bond replacements (pseudopeptides).