Amplification of Integrated Cloned Genes in Saccharomyces cerevisiae

克隆基因在微生物中的稳定性是 对于生产性重组细胞极其重要 流程. 该提案的目标包括确定 可能的放大程度和增强， 发生的时间尺度和频率，发展 细胞内蛋白质模型系统的研究 生产，确定对宿主菌株的影响， 稳定性研究和人口建模。 酵母 反转录转座子提供了一种独特的方法， 大量的整合克隆基因。 结果 从研究中应该可以证明这一点的潜力 多重稳定积分的唯一系统 克隆基因，这是一种方法，在许多情况下， 比更常用的质粒系统优越上级。

The stabilization of cloned genes in microorganisms is extremely important for productive recombinant cell processes. The goals of this proposal include determining the extent and enhancement of amplification possible, the time-scale and frequency on which it occurs, development and study of a model system for intracellular protein production, determining the effects on the host strain, stability studies, and population modeling. Yeast retrotransposons offer a unique approach for obtaining large numbers of integrated cloned genes. The results from the research should demonstrate the potential of this unique system for the stable integrations of multiple cloned genes, a method, which in many instances, should be superior to the more commonly utilized plasmid systems.