A Microscope and Cryostat for the Whitney Laboratory

这是为佛罗里达大学惠特尼海洋实验室购买用于组织学切片的光学显微镜和低温恒温器的建议。该设备特别用于海洋动物发育、分泌和信号传导的免疫细胞化学研究。这些研究采用了各种现代细胞和分子生物学技术，但由于任何发现最终都必须根据有关过程的细胞或细胞内定位进行评估，因此使用特定分子定位的技术变得越来越重要。这组研究人员要求获得资金，以购买(1)一台新的低温恒温器，以取代一台使用非常频繁但老化的设备，该设备的操作效率非常低且耗时长；(2)一台迄今为止实验室还没有的研究级复合显微镜。这些单元将被安置在目前正在建造的中央组织学/显微镜设施中。

This is a proposal to purchase an optical microscope and a cryostat for histological sectioning for use at the Whitney Marine Laboratory of the University of Florida. This equipment is particularly intended for immunocytochemical studies of development, secretion and signaling in marine animals. These studies employ of a variety of modern cell and molecular biological techniques, but since any findings must, ultimately, be evaluated with respect to the cellular or intracellular localization of the processes in question, the use of techniques for localizing specific molecules has become increasingly important. This group of investigators requests funds to acquire (1) a new cryostat to replace a very heavily used but aging unit whose operation is very inefficient and time consuming and (2) a research grade compound microscope which hitherto has not been available at the laboratory. These units will be housed in a central histology/microscopy facility that is currently being built.