Purchase of an LC-Electrospray Mass Spectrometer

该合同将帮助加州大学伯克利分校的化学学院购买配备有电喷雾电离能力和接口液体色谱仪的三重四极杆质谱仪。该仪器是化学系成员需要的，还将为分子生物系提供质谱学服务。该仪器的收购支持的研究领域包括：1)蛋白质结合色素的结构测定。2)荷电高分子量富勒烯衍生物的分析。3)新型合成生物聚合物的设计与合成。4)金属配位低聚糖的键合位置测定。5)蛋白质中非天然氨基酸的位点特异性掺入。6)稠电多离子化合物的分析。7)制备具有明确二级结构的多肽。8)转录因子的结构测定。9)核糖核酸分子的结构测定。质谱仪是用来测量分子的精确分子量的仪器。电喷雾装置是一种挥发样品的方法，以便将其引入质谱仪。需要这些信息，以便可以确定新合成化合物的化学成分，有时还可以确定其分子结构。伯克利工厂需要这种仪器，因为现有设备无法满足当前用户的需求。

This award will assist the College of Chemistry at the University of California Berkeley in the purchase of a triple quadrupole mass spectrometer equipped with electro- spray ionization capabilities and interfaced liquid chromatograph. The instrument is needed by members of the Department of Chemistry and will also provide mass spectroscopic services for the Department of Molecular Biology. Research areas supported by the acquisition of this instrument are: 1) Structure determination of protein-bound pigments. 2) Analysis of charged high molecular weight fullerene derivatives. 3) Design and synthesis of new classes of synthetic biopolymers. 4) Linkage position determination of metal-coordinated oligosaccharides. 5) Site specific incorporation of unnatural amino acids in proteins. 6) Analysis of densely charged poly-onium compounds. 7) Preparation of peptides with defined secondary structure. 8) Structure determination of transcription factors. 9) Structure determination of RNA molecules. %%% A mass spectrometer is an instrument used to measure the precise molecular weight of a molecule. The electrospray device is a way to volatilize the sample so that it may be introduced into the mass spectrometer. This information is needed so that the chemical composition and, sometimes, the molecular structure of newly synthesized compounds may be determined. An instrument of this description is required in the Berkeley facility because the current user needs cannot be meet with the existing equipment.