Development of Egg Organization During Oogenesis

发育过程中胚胎体计划的制定 许多动物的死亡是由于 卵子中的分子或细胞器 一个特别明显的例子是 是果蝇胚胎前部发育的特化。 bicoid基因的信使RNA位于前 在卵子发生过程中卵的边缘;受精后， Bicoid蛋白激活前特异性 合子基因 本建议中的实验针对 了解bicoid RNA如何在卵子发生过程中定位，包括 RNA的分子关联和细胞机制， 本地化 拟议的实验包括调查 分子间的相互作用导致bicoid RNA与 细胞骨架或其他大的细胞器，特别是 微管参与了这个过程。 的功能 吞下基因在卵子发生过程中bicoid RNA定位， 在实验中研究了吞咽蛋白质是否 与bicoid RNA相关，并确定功能重要的 部分吞咽蛋白质。 一组产妇的搜索 具有早期停滞表型的效应基因将被用于其他 其产物参与bicoid RNA定位的基因。 的 将通过以下方法监测bicoid RNA定位的时间过程： 诱导型RNA的构建和表达， 通过bicoid途径定位，允许测试的想法， bicoid RNA通过主动机制被转运到卵母细胞。

The establishment of the embryonic body plan during the development of many animals results from the asymmetric distribution of molecules or organelles in the egg. One particularly clear example is the specification of anterior development in Drosophila embryos. The messenger RNA of the bicoid gene is localized at the anterior margin of the egg during oogenesis; following fertilization the bicoid protein activates the expression of anterior-specific zygotic genes. Experiments in this proposal are directed toward learning how bicoid RNA is localized during oogenesis, including molecular associations of the RNA and the cellular mechanisms of localization. Proposed experiments include an investigation of molecular interactions that cause bicoid RNA to be associated with the cytoskeleton or other large organelles, particularly the involvement of microtubules in this process. The function of the swallow gene in bicoid RNA localization during oogenesis will be investigated in experiments to learn whether swallow protein is associated with bicoid RNA, and to identify functionally important parts of the swallow protein. A search of the group of maternal effect genes with early arrest phenotypes will be made for other genes whose products are involved in bicoid RNA localization. The time course of bicoid RNA localization will be monitored through the construction and expression of an inducible RNA that is localized via the bicoid pathway, allowing tests of the idea that bicoid RNA is transported to the oocyte by an active mechanism.