Molecular genetic dissection of plant disease resistance in Arabidopsis thaliana

拟南芥（Arabidopsis thaliana）是近年来研究植物病原相互作用分子遗传学的一个模式寄主系统。这使我们能够提出一系列明确的遗传实验来剖析植物抗病过程中识别和潜在信号转导事件的分子基础。我们建议通过鉴定和表征在抗病表达中改变的疾病易感突变体，对种族特异性和全身性获得性抗病进行遗传解剖。将对突变体进行表型和遗传表征，以确定在两种类型的抗性中有多少个位点控制抗病表达。我们的研究将集中在avrB特异性疾病易感突变体上，并将利用这些突变体通过染色体行走和/或T-DNA标记技术克隆RPG1抗病位点。在拟南芥中，突变可以很容易地分离出来，绘制基因图谱，和相应的野生型基因座的克隆，规避了在研究其他具有更复杂基因组的植物物种时遇到的许多缺点，使我们处于一个独特的位置，可以克隆RPG1抗病基因座，然后可以用来确定相同的抗病基因座是否存在于重要的商业作物中。

Arabidopsis thaliana has recently been developed as a model host system to study the molecular genetics if plant pathogen interactions. This has allowed us to propose a series of definitive genetic experiments to dissect the molecular basis of recognition and potential signal transduction events involved in plant disease resistance. We propose to genetically dissect both race-specific and systemic acquired disease resistance by identifying and characterizing disease susceptible mutants that are altered in the expression of disease resistance. The mutants will be phenotypically and genetically characterized to ascertain how many loci control the expression of disease resistance in both types of resistance. Our research will then focus on the avrB- specific disease susceptible mutants and will employ these mutants to clone the RPG1 disease resistance locus by the techniques of chromosome walking and/or T-DNA tagging. %%% The ease with which mutations in Arabidopsis can be isolated, genetically mapped, and corresponding wild type loci cloned circumvents many disadvantages encountered in the study of other plant species with more complex genomes and puts us in a unique position to clone the RPG1 disease resistance locus which can then be used to determine if the same resistance locus is present in commercially important crop plants.