Acquisition of an Automated DNA Sequencer

9221733夏威夷大学生物技术-分子生物学仪器和培训设施将使用雷默自动DNA测序仪进行研究，这些研究将包括对编码运动蛋白dynein的DNA进行表征；对线粒体DNA进行进化研究；以及对果蝇、海洋微生物物种和热带作物的重要遗传元件进行表征。来自该大学几个学校和研究机构的生物研究人员将使用该仪器对来自多个来源的特定基因座进行高度重复的测序，对特定结构基因进行广泛的测序，并对聚合酶链式反应扩增的“微卫星”DNA片段进行鉴定。自动DNA测序设备现在可以执行这一单调的任务，其精度与手动方法相当或更高，对人员的要求更低，而且不需要放射性材料。该仪器将以最低的费用向校园内的大约40个研究小组提供DNA测序服务。

9221733 Reimer Automated DNA sequencer will be used by the Biotechnology- Molecular Biology Instrumentation and Training Facility at the University of Hawaii in studies that will include characterization of the DNA that codes for the motility protein dynein; evolutionary studies on mitochondrial DNA; and characterization of significant genetic elements from Drosophila, marine microbial species, and tropical crop plants. Biological researchers from several schools and research institutes of the University will use the instrument for highly repetitive sequencing of specific loci from multiple sources, for extensive sequencing of specific structural genes, and for characterization of PCR amplified "micro-satellite" DNA fragments. Automated DNA sequencing equipment can now perform this monotonous task with comparable or higher accuracy than manual methods, with lower personnel requirements and without the need for radioactive materials. This instrument will provide access to DNA sequencing services to approximately 40 research groups on campus on a minimal fee basis.