Desorption Ionization Studies Using Fourier Transform Mass Spectrometry

这个项目是在一般地区的分析和表面 化学和质谱分析的子领域。 期间 这项为期三年的持续资助，罗素教授和 他的学生将应用基质辅助激光解吸 电离傅里叶变换质谱（MALDI/FTMS） 发展技术来调查结构和 大生物分子的反应性。 这项研究建立了 根据在NSF资助CHE-8821780的支持下获得的结果， 试图找到一种诱导序列特异性切割的方法， 多肽和多核苷酸离子。 教授的主要关注点 罗素的努力围绕着使用激发态气体 相金属离子作为一种手段，使这些大的生物分子， 感兴趣的是选择性地碎裂。 %%% 成功实现这项研究的目标将 为快速测定结构提供了一种简便的方法 复杂的肽和核酸在飞摩尔 集中制度。 这种分析方法是极端的 生物技术和临床化学进展的重要性。

This project is in the general area of analytical and surface chemistry and in the subfield of mass spectrometry. During the tenure of this three-year continuing grant, Professor Russell and his students will apply matrix assisted laser desorption ionization Fourier transform mass spectrometry (MALDI/FTMS) to the development of techniques to investigate the structure and reactivity of large biomolecules. This research, which builds upon results obtained under the aegis of NSF grant CHE-8821780, seeks to find a method for inducing sequence-specific cleavage of polypeptide and polynucleotide ions. A primary focus of Professor Russell's efforts centers around the use of excited state gas phase metal ions as a means to cause these large biomolecules of interest to fragment selectively. %%% The successful attainment of the goals of this research would provide a facile method for the rapid determination of structures of complex peptides and nucleic acids in the femtomole concentration regime. Such analytical methods are of extreme importance to advances in biotechnology and clinical chemistry.