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Enhanced Plasmid Stability Using Multiple Killer Genes

Enhanced Plasmid Stability Using Multiple Killer Genes
使用多个杀伤基因增强质粒稳定性
批准号：
9224864
负责人：
Thomas Wood
金额：
$4.73万
依托单位：
University of California-Irvine
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1993
资助国家：
美国
项目状态：
已结题
起止时间：
1993-08-15 至 1996-07-31
来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9224864&HistoricalAwards=false
关键词：
EnhancedPlasmidStabilityUsingMultiple

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中文摘要
翻译
9224864 Wood本提案是关于使用多个杀伤基因来提高质粒稳定性的研究。质粒稳定性的提高有助于在大肠杆菌中更高水平地表达重组蛋白产品，并为废物处理和生物修复提供有效的基因工程策略。要考察的两个要点包括：(1)介质组成和温度对质粒稳定性的作用；(2)使用两个杀伤基因增强稳定性的效果。将在恒温和摇瓶培养中进行实验。此外，还将执行一些建模。＊＊＊
英文摘要
9224864 Wood This proposal is on research to investigate enhanced plasmid stability by using multiple killer genes. Enhanced plasmid stability can be useful for a higher level of expression of recombinant protien products in E. coli and for effective genetic engineering strategies for waste treatment and bioremediation. The two main points to be examined include: (1) the role of media composition and temperature on plasmid stability; and (2) the effect of using two killer genes for enhanced stability. Experiments in both chemostat and shake - flask cultures will be performed. In addition, some modeling will also be performed. ***
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