High Performance Capillary Electrophoresis in the Undergraduate Biochemistry Laboratory

该项目将引入几个重要的生物分析 跨系本科生 生物化学实验室经验。 目前，学生 使用经典方法分离生物分子， 聚丙烯酰胺琼脂糖凝胶层析 电泳和离子交换树脂。 这些方法 在说明分离原理时是有用的;然而， 结果充其量是定性的。 一项技术 高效毛细管电泳（HPCE）， 它似乎具有多功能性，快速性， 简化集成到我们现有的五个 实验重点是分离，识别， 氨基酸、蛋白质和核酸的定量。 学院监督的本科生研究项目， 生物化学、生物学和化学也将使用HPCE 系统

The project will introduce several significant bioanalytical separations into the interdepartmental undergraduate biochemistry laboratory experience. At present, students are separating biomolecules using classical methods such as thin layer chromatography, polyacrylamide and agarose gel electrophoresis, and ion exchange resins. These methods are useful in illustration of the separation principles; however, the results are, at best, qualitative. A technology has been identified, high performance capillary electrophoresis (HPCE), which appears to have the versatility, quickness, and simplicity required for integration into five of our existing experiments that focus on separation, identification, and quantitation of amino acids, proteins, and nucleic acids. Faculty-supervised undergraduate research projects in biochemistry, biology, and chemistry will also employ the HPCE system.