Instrumentation for the Enhancement of Molecular Biology Program

该项目的目的是加强生物系本科生女性的重组DNA实验室体验。为满足发展计划的需要，需要购买四个仪器装置：(1)将使用DNA热循环仪，通过聚合酶链式反应(PCR)从真核细胞的cDNA文库中选择和扩增特定的真核基因。(2)将利用微型荧光计准确地测定DNA浓度。(3)电穿孔系统将使克隆的DNA在表达载体中高效转化细菌。(4)一个小型的电泳仪将被用来进行蛋白质的电泳和随后的免疫印迹以检测特定的蛋白质。

The aim of the project is the enhancement of the recombinant DNA lab experience for undergraduate women in the biology department. Funds are requested for the purchase of four instrumentation units to meet the needs of the development plan: (1) A DNA thermal cycler will be used to select and amplify a specific eukaryotic gene from a eukaryotic cDNA library by polymerase chain reaction (PCR). (2) A minifluorimeter will be utilized for accurate DNA concentration determinations. (3) An electroporation system will allow efficient transformation of bacteria with cloned DNA in an expression vector. (4) A mini electrophoresis system will be used to perform electrophoresis of proteins and subsequent Western blotting to detect specific proteins immunologically.