Glucuronide Plant Regulation

大肠杆菌β-葡糖醛酸糖苷酶基因（GUS）作为 用于分析转化植物中基因表达的融合标记 已经得到证实（Jefferson等人，1987）。合成，通过 一系列葡萄糖醛酸的化学和酶法 提出了植物调节物质的缀合物。在第一阶段， 我们将合成除草剂和毒素的结合物 并测试这些结合物特异性消融 表达GUS活性的组织和细胞。结合物将是 在组织培养和整个植株中测定了 以启动子依赖性方式引起局部细胞死亡。等 化合物将普遍用于植物研究， 控制发育和基因表达， 生产农业上重要的不育植物物种， 可逆的方式，通过使用选择性的应用程序， 底物和GUS-融合启动子选择。

Use of the Escherichia coli Beta-glucuronidase gene (GUS) as a fusion marker for analysis of gene expression in transform plants has been demonstrated (Jefferson, et al. 1987). The synthesis, by chemical and enzymatic methods, of a series of glucuronic acid conjugates of plant regulatory substances is proposed. In Phase I, we will be we will synthesize conjugates of herbicides and toxins and test these conjugates for the ability to specifically ablate tissues and cells expressing GUS activity. Conjugates will be assayed in tissue culture and in whole plants for the ability top cause localized cell death in a promoter dependent manner. Such compounds will be of general use for plant research into the control of development and gene expression and have the potential to produce agriculturally important sterile plant species in a reversible manner, through the use of selective application of substrate and choice of GUS-fusion promoter.