Molecular Systematics of the Chrysomeloides (Coleoptera)

9306667 FARREL将对植食性甲虫总科叶甲总科(叶甲科、天牛科、蚕蛾科)的32个亚科进行基于线粒体和核序列的系统发育研究。初步的序列数据已经证实了线粒体细胞色素氧化酶亚基I基因(COI)和核小核糖体亚基基因(18S)在实现这一目标方面的实用性。将进行系统发育分析，以确定每个甲虫科内的单系亚科之间的关系以及这些科本身的单系关系。主要目标是为反映系统发育的稳定分类提供基础。这将通过结合对拟议的分子数据集的分析和来自形态学的现有证据来实现。次要目标包括评估现有的几个假说，这些假说涉及最原始的亚科与某些寄主植物表面上的亲缘关系，以及最终测试这些甲虫谱系之间资源使用的变化对多样化比率的潜在影响。这个项目将使用形态信息和首席调查员从线粒体和核基因的DNA测序中获得的新数据来确定关系，并为这一大群甲虫建立健全的现代分类，这一大群甲虫包括3科32科。这一组或超家族包括72,000多种，其中许多是木材、农作物和储粮的重要害虫。该项目还将测试当前关于寄主植物从属关系变化对甲虫分裂速率的作用的想法。***

9306667 Farrell A phylogenetic study of the 32 subfamilies of the phytophagous beetle superfamily Chrysomeloidea (Chrysomelidae, Cerambycidae, Bruchidae) based on mitochondrial and nuclear DNA sequences will be done. Preliminary sequence data have confirmed the utility of the mitochondrial cytochrome oxidase subunit I gene (COI) and the nuclear small ribosomal subunit gene (18s) for accomplishing this goal. Phylogenetic analyses will be carried out to determine the relationships among the monophyletic subfamilies within each beetle family and the monophyly of the families themselves. The primary goal is to provide a basis for a stable classification that reflects phylogeny. This will be achieved by combining analyses of the proposed molecular data set with available evidence from morphology. Secondary goals include evaluation of several extant hypotheses concerning the ostensibly relictual affiliation of the most "primitive" subfamilies with certain hostplants and eventual tests of the potential influences of shifts in resource use among these beetle lineages on rates of diversification. %%% This project will use both morphological information and new data to be obtained by the Principal Investigator from DNA sequencing of both a mitochondrial and a nuclear gene to determine relationships and establish a sound modern classification for this large group of beetles which includes 32 families in 3 families. This group or superfamily includes over 72,000 species and many of them are important pests of timber, agricultural crops and stored grains. The project will also test current ideas on the role of shifts in hostplant affiliations on rates of beetle div ersification. ***