Designer Path for Guided Cell Migration and Development in Three Dimensional Tissue Culture Gels

9308527 Hu该项目旨在开发一种在三维结构（如凝胶）中为细胞迁移和组织发育创建定义路径的方法。 本研究的结果对组织工程和发育生物学具有重要意义。 组织工程学的一个应用是生产人造组织，例如。人工肝或胰腺组织。 光活化反应用于将细胞外基质或其他细胞粘附分子共价偶联至三维基质，以限定细胞迁移或发育的路径。 共价耦合的位置由两个激光束的交叉点限定。 分子沉积的尺寸、几何形状和轨迹可以通过控制每个激光束的扫描路径来产生。 ***

9308527 Hu This project is to develop a method of creating a defined path for cell migration and tissue development in three dimensional structures such as gels. The results of this research would be very important to tissue engineering and developmental biology. One application of tissue engineering would be the production of artificial tissue, eg. artificial liver or pancreas tissue. Photoactivated reactions are to be used to covalently couple extracellular matrix or other cell adhesion molecules to a three dimensional substratum to define the path for cell migration or development. The position of the covalent coupling is defined by the cross point of two laser beams. The size, geometry, and trajectory of molecular deposition can be generated by controlling the scanning path of each laser beam. ***