Reversible Immobilization of Enzymes and Bioligands Using Metal-Chelate Affinity Interactions

本研究的目的是研究和探索金属螯合相互作用的替代应用，并开发可逆和特异性的蛋白质分离方法。酶和抑制剂（生物配体）将偶联到可溶性双功能聚合物上，这些聚合物之前在一端用螯合化合物修饰。然后，这些修饰的螯合聚合物-生物配体将可逆地附着在固体或可溶性载体中先前固定的金属上。这种方法将允许通过简单地从系统中去除金属离子（以及随后的螯合聚合物-生物配体）来替换生物配体，从而使初始螯合基质保持完整。用这种方法，酶和特定抑制剂携带的螯合配体的固定金属可以可逆的方式连接。＊＊＊

9309326 Guzman The objective of this research is to study and explore alternative applications of metal chelate interactions, and to develop reversible and specific protein separation methods. Enzymes and inhibitors (bioligands) are to be coupled to soluble bifunctional polymers previously modified with a chelating compound at one of the ends. These modified chelating-polymer-bioligands will then be reversibly attached to a previously fixed metal in a solid or soluble support. This approach will allow the bioligands to be replaced whenever their activity is lost by simply removing the metal ion (and subsequently the chelate-polymer-bioligand) from the system, leaving the initial chelate matrix intact. With this approach, enzymes and specific inhibitors bearing a chelating ligand for the fixed metal can be attached in a reversible manner. ***