Engineering Research Equipment: A Photon Counting Spectrofluorometer for Studies in Materials Processing and Bioscience

9310932桑托雷这项研究设备赠款(REG)项目部分用于购买高分辨率光子计数分光荧光仪(SPEX FL 112)和所需的自制配件的材料。该仪器的主要作用将是为先进的材料加工和生物科学开发新的探针和实验技术；然而，它也将促进化学工程、分子生物学、乳液聚合物研究所、聚合物界面中心和分子生物学和生物科学中心的学生进行主要的荧光分析。开发新的探头需要一个内部样品池，一个全内反射剪切池，以及一个与外部实验的光学接口。这些配置专为快速交换而设计，为近期的几个项目提供了便利：(1)开发非辐射能量转移(NET)，以探测链段-表面接触的演变，并深入了解聚合物吸附和解吸的分子机制。(2)pH谱标尺的研制，用于监测吸附聚合物的缓慢松弛。(3)基于猝灭机制的均相免疫分析方法的建立及其与极化方法的比较。(4)酶在疏水表面的吸附及活性变化研究。(5)疏水改性梳状聚合物的溶液结构及其对流变学的影响；(6)溶胀、离子和水分传输以及物理老化对刚性基膜力学性能的影响。

9310932 Santore This research equipment grant (REG) project provides in part for the purchase of a high resolution photon counting spectrofluorometer (Spex FL 112) and for the materials for home- built accessories are requested. The instrument's main role will be in the development of novel probes and experimental techniques for advanced materials processing and biosciences; however, it will also facilitate workhorse fluorescence assays for students in Chemical Engineering, Molecular Biology, Emulsion Polymers Institute, Polymer Interfaces Center, and the Center for Molecular Biology and Biosciences. Novel probe development requires an internal sample cell, the construction of a total internal reflection shear cell, and an optical interface to external experiments. These configurations have been designed for rapid interchange, facilitating several projects in the immediate future: (1) Development of non- radiative energy transfer (NET) to probe the evolution of segment-surface contacts and provide insight into the molecular mechanisms for adsorption and desorption of polymers. (2) Development of a spectroscopic pH-ruler to monitor slow relaxations of adsorbed polymers. (3) Development of a homogeneous immunoassay relying on a quenching mechanism and comparison of this assay to one exploiting polarization. (4) Studies of enzyme adsorption and activity changes on hydrophobic surfaces. (5) Solution structures of hydrophobically-modified comb polymers and their impact on rheology and (6) The effect of swelling, ion and water transport, and physical aging on the mechanical properties of films on rigid substrates.