Convergence: RAISE Dynamic Touch-based Bacteria-Device Two-Way Communication

融合：RAISE动态触摸细菌-设备双向通信

• 批准号: 1848065
• 负责人: Maria Santore
• 金额: $ 97.5万
• 依托单位: University of Massachusetts Amherst
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-10-01 至 2024-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1848065&HistoricalAwards=false
• 关键词: Convergence; RAISE; Dynamic; Touch; based

Biological cells interact with their surroundings by sensing and responding to a range of stimuli and cues. In the tissues of higher organisms, cells communicate with each other, leading to collective functions. Research on electrical and mechanical communication of mammalian cells has led to progress in tissue engineering and to an understanding of the workings of nerve and muscle. Eventually, this understanding might translate into devices that could restore sight or motor activity. This research project will study how bacterial cells interact mechanically and electrically with the surface of an electronic device, how cell-cell interactions produce a response at the device surface, and how signals from a device can be transmitted by bacterial cells to other bacterial cells. The outcomes of these experiments could further knowledge about how touch-based bacterial communication can be utilized to engineer functional device-integrated communities of bacteria. These research efforts could nucleate a new discipline at the intersection of microbiology, biomaterials, and nanoelectronics. The creation of new devices that integrate bacteria could benefit society through advancing the manufacture of pharmaceuticals and specialty chemicals, smart logic-gated sensors, and bio-based filters for chemical remediation, energy conversion, and water purification. The research activities will add a new facet to workforce development and will foster a diverse community of scientists, internal and external to UMass, through team-based research, targeted workshops, and educational programs. The research team is committed to inspiring diverse K-12 students through the development of interdisciplinary teaching modules and weekend programs for high school students. This research project targets an understanding of how signals from a device or material are transmitted to and processed by bacteria, how intercellular communications can be intercepted and re-coded by devices, and how bacterial mechanotransduction and voltage responses play out at material interfaces. The program builds from a focus on device interactions with isolated cells to an understanding of how groups of cells interact with a device. Ultimately, the research will progress to a comprehension of the device interactions with an entire bacterial community. The project investigators will quantify timescales, length scales, and signal magnitudes relevant to mechanical and electrical interactions, targeting the basic principles of rapid bacterial-device communication. They will also address important issues in microbiology, including identifying changes in gene expression associated with the initial response to a given stimulus and in downstream single cell and group behaviors. This research project integrates cutting edge methods in microbiology, biomaterials, and nanoelectronics to target guiding scientific principles for engineering functional, device-integrated communities of bacteria. This research project also will impact the creation of new microscopy, prototype devices, and cellular reporters of gene activity that will more broadly advance science.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

生物细胞通过感知和响应一系列刺激和线索与周围环境相互作用。在高等生物的组织中，细胞相互交流，导致集体功能。对哺乳动物细胞的电和机械通信的研究导致了组织工程的进展，并使人们了解了神经和肌肉的工作原理。 最终，这种理解可能会转化为可以恢复视力或运动活动的设备。该研究项目将研究细菌细胞如何与电子设备的表面进行机械和电气相互作用，细胞与细胞的相互作用如何在设备表面产生响应，以及来自设备的信号如何通过细菌细胞传输到其他细菌细胞。 这些实验的结果可以进一步了解如何利用基于触摸的细菌通信来设计功能性设备集成的细菌群落。这些研究工作可能会成为微生物学、生物材料和纳米电子学交叉点的新学科的核心。创建集成细菌的新设备可以通过推进药物和特种化学品，智能逻辑门控传感器以及用于化学修复，能源转换和水净化的生物基过滤器的制造来造福社会。研究活动将为劳动力发展增加一个新的方面，并将通过基于团队的研究，有针对性的研讨会和教育计划，培养马萨诸塞大学内部和外部的多元化科学家社区。研究团队致力于通过为高中生开发跨学科教学模块和周末课程来激励不同的K-12学生。该研究项目的目标是了解来自设备或材料的信号如何传输到细菌并被细菌处理，细胞间通信如何被设备拦截和重新编码，以及细菌机械传导和电压响应如何在材料界面发挥作用。该计划从关注设备与孤立细胞的相互作用，到了解细胞群如何与设备相互作用。最终，研究将进展到理解设备与整个细菌群落的相互作用。项目研究人员将量化与机械和电气相互作用相关的时间尺度，长度尺度和信号幅度，目标是快速细菌-设备通信的基本原理。他们还将解决微生物学中的重要问题，包括识别与对给定刺激的初始反应以及下游单细胞和群体行为相关的基因表达变化。该研究项目整合了微生物学，生物材料和纳米电子学的尖端方法，以指导工程功能，设备集成细菌群落的科学原则。该研究项目还将影响新的显微镜、原型设备和基因活动的细胞报告者的创建，这些将更广泛地推动科学。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Depletion forces drive reversible capture of live bacteria on non-adhesive surfaces

消耗力驱动非粘性表面上活细菌的可逆捕获

• DOI: 10.1039/d1sm00631b
• 发表时间: 2021
• 期刊: Soft Matter
• 影响因子: 3.4
• 作者: Niu, Wuqi Amy;Rivera, Sylvia L.;Siegrist, M. Sloan;Santore, Maria M.
• 通讯作者: Santore, Maria M.

Surface Chemistry Guides the Orientations of Adhering E. coli Cells Captured from Flow

表面化学指导从流中捕获的粘附大肠杆菌细胞的方向

• DOI: 10.1021/acs.langmuir.1c00764
• 发表时间: 2021
• 期刊: Langmuir
• 影响因子: 3.9
• 作者: Xu, Zhou;Niu, Wuqi Amy;Rivera, Sylvia L.;Tuominen, Mark T.;Siegrist, M. Sloan;Santore, Maria M.
• 通讯作者: Santore, Maria M.