Acquisition of a Shared Imaging Plate Area Detector Diffractometer

请求提供资金购买一个成像板面积探测器系统，以便测量生物大分子晶体的X射线衍射数据。 该系统将直接支持3名主要调查员和4名次要调查员的研究。 这些研究人员需要立即收集11种蛋白质和复合物的26种不同现有晶体形式的数据。 由于这些晶体包括蛋白激酶、蛋白磷酸酶、DNA甲基化酶、DNA核酸内切酶、参与神经突延伸的钙结合蛋白、锌酰胺水解酶和参与糖酵解和神经元生成控制的酶，这些晶体结构的阐明将推进DNA甲基化、蛋白磷酸化、哺乳动物发育、细胞生长控制、基因表达、和细胞分化，并提供对癌症，糖尿病和阿尔茨海默氏病等人类疾病的基本机制的见解。 这些晶体中的大多数需要大的有效面积，高动态范围和/或低噪声的成像板面积检测器衍射仪提供，此外，该设备将用于教学的高级课程，在大分子晶体学提供每年10月在冷泉港实验室（CSHL）和在夏季培训选定的本科生在结构生物学。 新仪器将使我们继续在我们先进的教学计划中为学生提供出色的培训。

Funds are requested for the purchase of an imaging plate area detector system in order to measure x-ray diffraction data from biological macromolecular crystals. This system would directly support the research of 3 major investigators and 4 minor investigators. These investigators have immediate data collection needs for 26 different existing crystal forms of 11 proteins and complexes. Since these crystals include protein kinases, protein phosphatases, DNA methylases, DNA endonucleaes, a calcium binding protein involved in neurite extension, a zinc amidohydrolase, and an enzyme involved in the control of glycolysis and gluconeogenesis, the elucidation of these crystal structures will advance the knowledge of the DNA methylation, protein phosphorylation, mammalian development, control of cell growth, gene expression, and cell differentiation and provide insights into the basic mechanism of the human afflictions of cancer, diabetes and Alzheimer's disease. Most of these crystals require the large active area, high dynamic range and/or low noise that the imaging plate area detector diffractometer provides, In addition, the equipment will be used in teaching an advanced course in macromolecular crystallography offered each October at Cold Spring Harbor Laboratory (CSHL) and in training selected undergraduates in structural biology during the summer. The new instrument will let us continue to give outstanding training to students in our advanced teaching program.