IMAGING SHARED RESOURCE

影像共享资源为Wistar研究所癌症中心成员提供高端 显微镜和小动物成像服务。近年来，技术进步使其 可能利用定量、高分辨率成像方法来剖析时空要求 与恶性转化、转移性播散和对治疗的抗性相关的细胞行为。 在本申请的最后一个项目期间，本资源经历了重大转变， 新的科学领导和技术能力的显着扩展需要支持一个 广泛的实验成像。成像资源现在提供宽视野，直立和倒置 显微镜，低放大率成像，特写和微距摄影，活细胞延时成像，激光 扫描共聚焦成像、双光子显微镜和小动物全身成像。 在上一个资助期间，癌症中心做出了重大努力来升级资源， 投资超过60万美元，打造配备Leica TCS SP5 II扫描激光器的全新共聚焦套件 共聚焦显微镜经验丰富的工作人员可与研究人员就实验性 设计、成像技术和采集后分析，以提供设备的最佳结果， 技术.工作人员直接与用户合作获取他们的图像，或培训用户操作 设备独立。其他服务包括开发自定义图像分析宏， 负责维护成像设备，并协助和培训图像编辑软件以供出版。 该资源还进行了技术复杂的光漂白测定实验，3D和4D 跟踪.该资源强调图像处理中的道德实践，以及图像的各个方面 采集、调整和分析。该资源积极与其他癌症中心合作共享 帮助用户使用高通量成像模式的资源，包括， 例如，与流式细胞术资源合作的Amnis ImageStream，以及IVIS 2001 小动物成像仪与动物资源合作。成像被归类为I类共享 资源，以反映其服务的明确定义，基本性质。该分类在 本申请的癌症中心管理部分。在过去的项目期间。癌症中心 所有三个计划的成员都利用了资源的服务， 初步数据大大提高了科学出版物和赠款提交的优先级。

The Imaging Shared Resource provides Wistar Institute Cancer Center members access to high end microscopy and small animal imaging services. In recent years, technological advances have made it possible to utilize quantitative, high resolution imaging approaches to dissect spatio-temporal requirements of cellular behavior related to malignant transformation, metastatic dissemination and resistance to therapy. During the last project period of this application, this Resource has undergone a major transformation with new scientific leadership and a significant expansion of the technologic capabilities needed to support a broad array of experimental imaging. The Imaging Resource now offers widefield, upright and inverted microscopy, low magnification imaging, close-up and macro photography, live-cell time lapse imaging, laser scanning confocal imaging, 2-photon microscopy, and small animal whole body imaging. During the last funding period the Cancer Center has made a significant effort to upgrade the Resource, investing over $0.6 million to create a new confocal suite equipped with a Leica TCS SP5 II scanning laser confocal microscope. Highly experienced staff are available to consult with investigators on experimental design, imaging techniques, and post-acquisition analysis to provide optimal results from equipment and techniques. Staff members work directly with users to acquire their images, or train users to operate the equipment independently. Other services include developing custom image analysis macros, assistance with maintaining imaging equipment and assistance and training with image editing software for publication. The Resource also carries out technically complex photobleaching assay experiments, and 3D and 4D tracking. The Resource stresses ethical practices in image manipulation, as well as in all aspects of image acquisition, adjustment and analysis. The Resource actively collaborates with other Cancer Center Shared Resources to assist users with high-throughput imaging modalities available in those Resources, including, for example, the Amnis ImageStream in collaboration with the Flow Cytometry Resource, and the IVIS 2001 small animal imager in collaboration with the Animal Resource. Imaging was classified as a Type I Shared Resource to reflect the well-defined, essential nature of its services. This classification is described in the Cancer Center Administration section of this application. During the past project period. Cancer Center members from all three Programs have leveraged the services of the Resource and generated critical preliminary data that considerably increased the priority of scientific publications and grant submissions.