Molecular Biophysics of Protein Adsorption in Ion-Exchange Chromatography

本研究的目的是发展分子热力学模型来预测蛋白质在离子交换色谱中的吸附。模型将结合静电和范德华成分的蛋白质表面和蛋白质相互作用与吸附模式和分子取向有关的特别利益。为了检验动力学效应，将进行布朗动力学模拟。一个互补的实验组成部分包括通过全内反射荧光（TIRF）光谱、表面力测量、原子力显微镜和其他方法表征蛋白质在石英或二氧化硅上的吸附。本项目将有助于改进某些药物和基因工程技术产品纯化的主要方法之一。

The aim of this research is the development of molecular thermodynamic models to predict protein adsorption in ion exchange chromatography. Models will incorporate electrostatic and van der Waals components for protein-surface and protein-interactions with particular interests related to adsorption patterns and molecular orientations. Brownian dynamics simulations are to be performed in order to examine kinetic effects. A complementary experimental component comrises characterization of protein adsorption on quartz or silica by total internal reflectance fluorescence (TIRF) spectroscopy, surface force measurements, atomic force microscopy and other methods. This project should help improve one of the major methods for purification of some pharmaceuticals and products of genetic engineering technologies.