Molecular Genetics of Fission Yeast Course

9405372 Grodzicker这笔赠款支持将于1994年至1998年秋季举办的冷泉港实验室课程--分子遗传学裂变酵母。这门课程提供了一个短期的，两周的强化训练，学习如何使用分裂酵母作为实验有机体的经典方法和分子方法。这门课程的目的是让学生做好准备，利用先进和/或专门的分子遗传学、经典遗传学和生物化学方面的技术和概念进行研究，这些技术和概念可应用于许多研究，包括细胞周期调节和生长控制。重点不仅放在技术上，而且放在解决实验问题的方法上。组织这门课程的目的是在三个主要领域指导学生。在第一门课程，即分裂酵母的细胞生物学中，学生将获得在显微镜下使用荧光探针的专业知识。此外，还介绍了细胞周期分析的最新技术，如流式细胞术和通过淋洗进行细胞同步。生物化学实验也被提出，使学生能够连接遗传和生化数据，并使他们准备将这些方法与他们自己的工作联系起来。该课程还提供了在酵母中可能进行的强大的经典遗传分析方面的科学专业知识。学生学习识别突变基因、绘制基因图谱和四分体分析的方法。最后，学生获得了分子遗传学方面的专业知识。学生在酵母研究中使用重组DNA技术的理论和实践方面的培训。教师是根据他们对该领域的贡献和知识来选择的。教职员工邀请那些放弃当前研究的最新报告的讲师。讲师们都在各自的领域做出了重大贡献。学员的范围从研究生到高级调查人员，由课程教职员工挑选。这笔赠款支持1994-1998年秋季在冷泉港实验室举行的为期两周的分裂酵母分子遗传学课程。由于课程持续时间较短，高级和初级人员可以在远离其他时间和注意力要求的环境中参加并接受短期、高强度的培训。该课程为科学家提供了一个不同寻常的机会，让他们可以在另一个专业进行再培训，或者将一个新领域的工作应用到他们自己的研究兴趣中。***

9405372 Grodzicker This grant supports the Cold Spring Harbor Laboratory course, The Molecular Genetics Fission Yeast, to be held in the fall of 1994-1998. The course provides a short, two week, intensive training in classical and molecular approaches to the use of fission yeast as an experimental organism. It is designed to prepare the student to enter directly into research that makes use of advanced and/or specialized techniques and concepts in molecular genetics, classical genetics and biochemistry that can be applied to many studies, including cell cycle regulation and growth control. The emphasis is placed not only on techniques but on the approaches to experimental problems. The course is organized to instruct students in three major areas. In the first, which is the cell biology of fission yeast, students gain expertise in the use of fluorescent probes for microscopy. In addition, state of the art techniques for cell cycle analysis, such as flow cytometry and synchronization of cells by elutriation are presented. Biochemical experiments are also presented to enable students to connect genetic and biochemical data and prepare them to relate these approaches to their own work. The course also provides scientific expertise in the powerful classical genetic analyses possible in yeast. Students learn approaches to identifying mutant genes, mapping genes and to tetrad analysis. Finally, students gain expertise in molecular genetics. Students are trained in the theory and practice of the use of recombinant DNA technology in studies in yeast. The faculty are chosen on the basis of their contributions to and knowledge of field. The faculty invite lecturers who give up-to-the-minute reports on current research. The lecturers have all made significant contributions to their fields. The trainees range from graduate students to senior investigators, and are chosen by the course faculty. %%% This grant supports an annual two-week course in the molecular genetics of fission yeast at Cold Spring Harbor Laboratory in the fall of 1994-1998. Because of the short duration of the course, senior, as well as junior individuals can attend and receive a short, intense period of training in an environment remote from other demands on their time and attention. The course provides an unusual opportunity for scientists to retrain in another speciality or to apply the work of a new field to their own research interests. ***