National High Field FT-ICR Mass Spectrometry Facility

摘要 提案：9413008 PI：马歇尔，A. G. 研究机构：佛罗里达州立大学 化学研究仪器和设施计划的这一奖项将有助于佛罗里达州立大学国家高磁场实验室的FT-ICR-质谱设施的发展。 使用该仪器进行的研究包括以下内容：直接测序，化学反应性和气相蛋白质的形状分析，抗体大小（150，000 Da）;直接光学吸收光谱和电子顺磁共振质量选择捕获离子;和常规直接鉴定原油复杂混合物的未分离组分。 该设施将拥有世界上最高场的离子回旋共振质谱仪。 FT-ICR的技术发展将对生物和分析化学产生重大影响，NSF中心将促进这些发展向广泛的用户社区的传播。 该设施的方法开发将包括以下方面的研究： a）内部和外部电离技术， B）用于不耐热和/或固体化合物的离子源， c）IR激光解吸（LD）和 d）高沸点混合物的汽化。 内部核心小组研究将关注FT-ICR-质谱的应用，以： a）肽和蛋白质测序， B）蛋白质中的氢-氘交换， c）磷脂鉴定和结构分析， d）质量选择捕获离子的EPR光谱 e）用于半导体中的元素分析和深度剖析的辉光放电FT-ICR，以及 f）捕获离子的光谱学。 用户设施：在第一年，50%的仪器时间将提供给佛罗里达州立大学以外的用户，在随后的几年中增加到80%。 教育推广：该设施将为学生和学术界和工业界的其他科学家提供一个出色的培训基地。 该计划将包括大量的研究生和博士后学生，以及客座教授和工业科学家。 实习生项目将覆盖高中和本科学生和教师。 特别包括女性和少数群体的代表。 傅里叶变换离子回旋共振（FT-ICR）质谱法是用于在不存在溶剂的情况下（即，气相）。 应用范围从蛋白质结构到原油。

ABSTRACT PROPOSAL: 9413008 PI: MARSHALL, A. G. INSTITUTION: FLORIDA STATE UNIVERSITY This award from the Chemistry Research Instrumentation and Facilities Program will aid in the development of FT-ICR-Mass Spectrometry Facility in the National High Magnetic Field Laboratory at Florida State University. The research to be performed with this instrumentation include the following: direct sequencing, chemical reactivity, and shape analysis of gas-phase proteins up to antibody size (150,000 Da); direct optical absorption spectroscopy and electron paramagnetic resonance of mass-selected trapped ions; and routine direct identification of unseparated components of mixtures as complex as crude oil. This Facility will have the highest-field ICR mass spectrometers in the world. The technological development in FT-ICR will have major impact in biological and analytical chemistry, and the NSF center will enhance the spread of these development to a broad community of users. Methods Development at the facility will include research on: a) internal and external ionization techniques, b) ion Sources for thermolabile and/or solid compounds, c) IR laser desorption (LD) and d) vaporization of high boiling point mixtures. In-house core group research will be concerned with applications of FT-ICR-mass spectrometry to: a) peptide and protein sequencing, b) hydrogen-deuterium exchange in proteins, c) phospholipid identification and structural analysis, d) EPR spectroscopy of mass-selected trapped ions e) glow discharge FT-ICR for elemental analysis and depth profiling in semiconductors, and f) optical spectroscopy of trapped ions. User Facility: In the first year 50% of instrument time will be available to users outside Florida State University, increasing to 80% in subsequent years. Education Outreach: The facility will provide an outstanding training ground for st udents and other scientists in academic and industry. The program will include a large number of graduate and postdoctoral student, and visiting professors and industrial scientists. Intern programs will reach high school and undergraduate students and teachers. Representation by female and minority groups are specifically included. Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry is the most versatile technique for unscrambling and quantifying ion-molecule reaction kinetics and equilibria in the absence of solvent (i.e., the gas phase). Applications, range from protein structure to crude oil.