The Molecular Basis for Variation in Heart Glucose Metabolism

小行星9419781 心脏中的代谢利用葡萄糖作为能量来源。 葡萄糖代谢受分解这种营养素的酶的浓度影响（即，糖酵解途径中的酶）。 Crawford博士打算测量葡萄糖利用率、糖酵解途径中所有酶的酶浓度变化，并确定导致酶浓度变化的分子机制。 为了探索这些过程，将利用硬骨鱼（Fundulus heteroclitus），一种生活在不同热环境中的冷血生物。 该物种的种群应该改变心脏代谢以适应这些不同的环境。 这些修饰应该揭示调节葡萄糖使用的机制。 他打算通过改变心肌细胞中一种或多种糖酵解酶的浓度来实证验证这些结果，并量化对葡萄糖利用的影响。 这种方法的数据将有助于了解基因表达在代谢过程中的作用，并提供经验证据解决有关代谢控制的问题。 ***

9419781 Crawford Metabolism in the heart utilizes glucose as a source of energy. Glucose metabolism is affected by the concentrations of the enzymes that break down this nutrient (i.e., enzymes in the glycolytic pathway). Dr. Crawford intends to measure the rate of glucose utilization, the variation in enzyme concentration for all enzymes in the glycolytic pathway and determine the molecular mechanisms responsible for the change in enzyme concentration. To explore these processes, the teleost fish (Fundulus heteroclitus), a cold-blooded organism that lives in different thermal environments, will be utilized. Populations of this species should modify heart metabolism to adapt to these different environments. These modifications should reveal mechanisms that regulate glucose use. He intends to empirically verify these results by altering the concentration of one or more glycolytic enzymes in cardiac muscle cells and quantify the effect on glucose utilization. The data from this approach will contribute to knowing the role of gene expression in metabolic processes as well as provide empirical evidence addressing questions concerning metabolic control. ***