Acquisition of a Dedicated TOF-MALDI Protein Mass Spectrometer

要求提供资金，以购置飞行时间、基质辅助激光解吸电离质谱仪，用于分析蛋白质、共价蛋白质-配体复合物、糖肽、磷酸肽、含有非蛋白质氨基酸的设计肽、光亲和改性肽和蛋白质后改性蛋白质的酶促降解物。项目将由来自化学，生物化学细胞生物学，过敏，流变学临床免疫学和生理学生物物理学部门的七名主要教师用户进行，他们的研究需要分析修饰肽。该仪器将被安置在生理学生物物理学系的大分子分析和合成中心，并作为用户可访问、操作员维护的共享设施运行。生物技术中心，一个纽约州先进技术中心将为该仪器提供人员配备和行政监督。

Funds are requested for acquisition of a time-of-flight, matrix-assisted laser desorption ionization mass spectrometer (TOF-MALDI-MS) for analysis of proteins, covalent protein-ligand complexes, glycopeptides, phosphopeptides, designed peptides with nonprotein amino acids, photoaffinity modified peptides, and enzymatic digests of posttranslationally-modified proteins. Projects will be conducted by seven major faculty users from the departments of Chemistry, Biochemistry & Cell Biology, Allergy, Rheumatology & Clinical Immunology, and Physiology & Biophysics whose research requires analysis of modified peptides. The instrument will be housed in the Center for the Analysis and Synthesis of Macromolecules in the Department of Physiology & Biophysics and operated as a user-accessible, operator-maintained shared facility. The Center for Biotechnology, a New York State Center for Advanced Technology will provide staffing and administrative oversight for the instrument.