P-Protein Mutants: Tools to Analyze Phloem Protein Function

9422615汤普森韧皮部组织在碳水化合物、激素、氨基酸和其他对植物生长至关重要的有机化合物的长途运输中起着核心作用。进一步了解韧皮部分化过程中结构和生理变化的分子基础，将为开发提高植物生长和生产力的创新方法提供基础。P-蛋白是大多数植物筛分分子中的一个整体成分，但这些蛋白在韧皮部发育和功能中的意义尚未得到实验验证。这项拟议项目的最终目标是确定两个P蛋白，PP1和PP2在韧皮部生理中的作用(S)。汤普森博士将验证这一假说，即P蛋白细丝插入受伤的筛子元件，使筛子管内保持膨大，以保持韧皮部的功能完整性。这些实验还旨在评估P-蛋白在未受伤植物韧皮部分化过程中的生理作用。

9422615 Thompson The phloem tissue plays a central role in the long-distance transport of carbohydrates, hormones, amino acids, and other organic compounds that are essential for plant growth. A further understanding of the molecular basis for the structural and physiological changes that occur during phloem differentiation will provide a basis for the development of innovative methods to improve plant growth and productivity. P-proteins are an intergral component of sieve elements in most plants, however the significance of these proteins in phloem development and function has not been experimentally tested. The ultimate goal of this proposed project is to determine the role(s) of two P- proteins, PP1 and PP2, in phloem physiology. Dr. Thompson will test the hypotheqis that P-protein filaments plug wounded sieve elements maintaining turgor within the sieve tube to preserve the functional integrity of the phloem. The proposed experiments are also designed to evaluate physiological roles for P-proteins during phloem differentiation in unwounded plants.