Enhanced Gene Expression in Immobilized Whole-Cell Biocatalysis

9424063 Flickinger该项目是研究固定细胞的聚合物技术以及饥饿条件下受限细胞的行为。 固定化系统由三层组成：无孔支持物;细胞和聚合物;以及薄的覆盖皮肤。 制备后的细胞活力 以及不同基因的表达、信息稳定性等。 还将对基质的形态进行表征，并用于改善生物学结局。 研究的主要目标是最大化固定化细胞的活力。 此外，还将检查通过添加营养物引起的细胞周期性复苏。 ***

9424063 Flickinger This project is on research to study the polymer technology of immobilizing cells as well as the behavior of confined cells under starvation conditions. The immobilization system consists of three layers: a non porous support; cells and polymer; and a thin covering skin. Cell viability after preparation and storage, as well as the expression of different genes, message stability, etc. will be investigated. The morphology of the matrix will also be characterized and used to improve the biological outcome. A major goal of the research is to maximize the viability of the immobilized cells. Also, the periodic revival of the cells by nutrient addition will be examined. ***