The Cell Biology and Genetics of the Maize Meiotic Kinetochore

;​R o o t E n t y F C o m p o b j b W o r d d o c u m e n t - O b j e c t P O O l/ 0 1 2 3 4 5 6 7 8 9F Microsoft Word 6.0文档MSWordDoc。6;​这是一项关于玉米减数分裂或配子产生的细胞遗传学研究，重点是结构，着丝点，减数分裂纺锤体附着在染色体上的地方。在作为该建议基础的博士后工作中，使用现已商品化的反褶积软件对荧光染色体进行图像处理，从而更准确地确定了减数分裂期间染色体配对的时间，并绘制了减数分裂开始时玉米染色体的新细胞学图谱。利用玉米突变自交系，获得了证据支持着丝点是减数分裂纺锤体形成的先决条件的假设，并且在10号染色体异常的自交系中出现了着丝点样结构。Dawe博士现在将追求更高分辨率的成像，以实现着丝点本身的三维重建。用于荧光标记着丝点的抗体现在将用于绘制其亚结构。克隆这些着丝点蛋白的编码蛋白，制备单克隆抗体。这些试剂和选择的自交系将用于绘制减数分裂和有丝分裂着丝点之间的结构差异，参与还原分裂和赤道分裂的着丝点之间的结构差异，以及正常着丝点和在异常10号染色体中发现的着丝点样结构之间的结构差异。着丝点是染色体上最重要的结构之一，从历史上看，减数分裂着丝点为着丝点的广泛功能提供了重要的见解。它已被证明结合端粒DNA，将姐妹染色单体结合在一起，结合纺锤体微管，与纺锤体上的分子马达相互作用，并产生用作细胞周期检查点的信号。这项研究剖析了着丝点的组成，并展示了它们是如何相互作用的，以及它们是如何随时间发生空间变化的。*** ;哦+ 0 $ H l y S u m m r I f n o r m t I o n ( .​D h R:\WWUSER\TEMPLATE\NORMAL。尤娜·所罗门尤娜·所罗门@ 0 @ @ 0 @ f#微软Word 6.0 2;e3e - p p p p p p p p p p p p p p p p p p p p p p p p p p这是一项关于玉米减数分裂或配子产生的细胞遗传学研究，重点研究减数分裂纺锤体附着在染色体上的着丝点结构。在作为该建议基础的博士后工作中，使用现已商品化的反褶积软件对荧光染色体进行图像处理，从而更准确地确定了减数分裂期间染色体配对的时间，并绘制了减数分裂开始时玉米染色体的新细胞学图谱。利用玉米突变自交系，获得了证据

; R o o t E n t r y F C o m p O b j b W o r d D o c u m e n t - O b j e c t P o o l / 0 1 2 3 4 5 6 7 8 9 F Microsoft Word 6.0 Document MSWordDoc Word.Document.6 ; 95-05670 R. Kelly Dawe This is a cytogenetic study of meiosis, or gamete production, in maize, focusing on the structure, the kinetochore, where the meiotic spindle attaches to the chromosome. In the post-doctoral work which serves as a basis for this proposal, image processing of fluorescent chromosomes using deconvolution software now comercially available resulted in more accurate timing of when chromosome pairing occurs during meiosis and a new cytological map for maize chromosome at the start of meiosis. Using mutant maize inbred lines, evidence was obtained to support the hypothesis that the kinetichore is a prerequisite to spindle formation in meiosis and that kinetochore-like structures appear in an inbred line with an abnormal chromosome 10. Dr Dawe will now pursue higher resolution imaging in order to achieve 3D reconstruction of the kinetochore itself. Antibodies which have been used to fluorescently tag the kinetochore will now be used to map its substructure. The proteins which code for these kinetochore proteins will be cloned and monoclonal antibodies prepared. These reagent s and select inbred lines will be used to map the structural differences between the meiotic and mitotic kinetochores, between kinetochores involved in reductional vs. equatorial division, and between the normal kinetochore and the kinetochore-like structure found in a line with abnomral chromosome 10. %%% The meiotic kinetochore has historically provided important insights into the broader functions of the kinetochore, one of the most important structures on the chromosome. It has been shown to bind telomeric DNA, hold sister chromatids together, bind to spindle microtubules, interact with molecular motors on the spindle,and produce a signal that is used as a cell cycle check point. Insight into the way it does this will come with this study which dissects the components of the kinetochore and shows how they interact and change spacially with time. *** ; Oh +' 0 $ H l S u m m a r y I n f o r m a t i o n ( . D h R:\WWUSER\TEMPLATE\NORMAL.DOT 95-05670 Una Solomon Una Solomon @ O @ @ O @ F # Microsoft Word 6.0 2 ; e 3 e - p p p p p p p u 1 7 W T 3 u p u p p p p p p p p i 95-05670 R. Kelly Dawe This is a cytogenetic study of meiosis, or gamete production, in maize, focusing on the structure, the kinetochore, where the meiotic spindle attaches to the chromosome. In the post-doctoral work which serves as a basis for this proposal, image processing of fluorescent chromosomes using deconvolution software now comercially available resulted in more accurate timing of when chromosome pairing occurs during meiosis and a new cytological map for maize chromosome at the start of meiosis. Using mutant maize inbred lines, evidence was obtai