Molecular Structure and Function of the Arabidopsis RPM1 Disease Resistance Gene

详细了解导致植物抗病的分子事件具有重要的现实意义和基础意义。该建议采用互补的遗传和细胞生物学方法来剖析模式植物拟南芥中一种称为RPM1的特定抗性基因的结构和功能。继续分析将确定表达rpm1依赖性疾病抗性所需的新基因。研究的四个目标领域是：1。1 . RPM1蛋白的结构功能分析；它的亚细胞定位和拓扑结构；3. 与之相互作用的其他植物蛋白的数量和性质；4. 其功能所需的其他基因座的遗传鉴定。人们早就认识到，植物中含有的基因，其功能是由感染病原体的特定分离株中特定和相应基因的存在所触发的。这种“基因对基因”的相互作用负责激活一系列生化和细胞事件，导致疾病抗性状态。此外，最初的识别事件可诱导更持久的全身性和病原体非特异性免疫，称为全身性获得性抵抗。近年来，一些植物抗病基因的克隆，以及引发这些基因的病原基因的克隆，预示着对植物抗病的分子和细胞反应的理解将有根本性的突破。其中一个来自模式植物拟南芥的基因RPM1将在分子和细胞水平上进行分析。该基因编码的蛋白质将被分析其结构功能、亚细胞定位和拓扑结构，以及与之相互作用的其他植物蛋白的数量和性质。将寻找其功能所需的其他遗传位点。＊＊＊

9505678 Dangl A detailed understanding of the molecular events leading to plant disease resistance is of both practical importance and basic interest. This proposal takes complementary genetic and cell biological approaches to dissect the structure and function of a particular resistance gene, called RPM1, from the model plant Arabidopsis. Continuing analysis will identify new genes required for the expression of RPM1-dependent disease resistance. The four target areas of research are: 1. structure-function analysis of the RPM1 protein, 2. its subcellular localization and topology; 3. the number and nature of other plant proteins with which it interacts and ; 4. genetic identification of other loci required for its function. %%% It has long been recognized that plants contain genes whose function is triggered by the presence of specific and corresponding genes in particular isolates of infecting pathogens. This "gene for gene" interaction is responsible for activation of the array of biochemical and cellular events leading to a disease resistant state. In addition, the initial recognition event can induce a longer lasting, systemic and pathogen non-specific immunity" termed systemic acquired resistance. The recent cloning of several plant disease resistance genes, and the corresponding pathogen genes which trigger their action, presages fundamental breakthroughs in understanding the molecular and cellular responses of plant disease resistance. One such plant gene, RPM1, from the model plant, Arabidopsis, will be analyzed at the molecular and cellular level. The protein encoded in the gene will be analyzed for its structure-function, its subcellular localization and topology and the number and nature of other plant proteins with which it interacts. Other genetic loci required for its function will be sought. ***